Expression of I-A(α)(k) and allelic restriction on A(α)/A(β) pairing in transgenic mice

J. Martin, B. Y. Wei, S. Savarirayan, C. S. David

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Cell surface expression of class 11 major histocompatibility complex encoded (1a) molecules depends on association of the component α and β chain into a stable heterodimer. Studies on cell lines transfected with MHC class II genes have revealed important limitations on the assembly of haplotype-mismatched A(α):A(β) complex. In order to study α:β chain pairing restriction in vivo a number of lines of transgenic mice carrying the A(α)(k) gene were generated. The transgene was studied in the context of H-2b, H-2(s), H-2(q), H-2(u), H-2(f) and H-2(v) haplotypes. Initial FACS analysis of spleen and peripheral blood cells showed no A(α)(k) expression. Spleen cells stimulated with LPS and analysed by FACS showed A(α)(k) expression in three different H-2b strains as well as H-2(u), but not in others. These data indicate that A(α)(k) can associate with A(β)(b) and A(α)(k), but not with A(β)(s), A(β)(q), A(β)(f), A(β)(v).

Original languageEnglish (US)
Pages (from-to)151-159
Number of pages9
JournalJournal of Immunogenetics
Volume17
Issue number1-2
StatePublished - 1990

Fingerprint

Haplotypes
Transgenic Mice
Spleen
MHC Class II Genes
Major Histocompatibility Complex
Transgenes
Blood Cells
Cell Line
Genes

ASJC Scopus subject areas

  • Immunology
  • Genetics

Cite this

Martin, J., Wei, B. Y., Savarirayan, S., & David, C. S. (1990). Expression of I-A(α)(k) and allelic restriction on A(α)/A(β) pairing in transgenic mice. Journal of Immunogenetics, 17(1-2), 151-159.

Expression of I-A(α)(k) and allelic restriction on A(α)/A(β) pairing in transgenic mice. / Martin, J.; Wei, B. Y.; Savarirayan, S.; David, C. S.

In: Journal of Immunogenetics, Vol. 17, No. 1-2, 1990, p. 151-159.

Research output: Contribution to journalArticle

Martin, J, Wei, BY, Savarirayan, S & David, CS 1990, 'Expression of I-A(α)(k) and allelic restriction on A(α)/A(β) pairing in transgenic mice', Journal of Immunogenetics, vol. 17, no. 1-2, pp. 151-159.
Martin, J. ; Wei, B. Y. ; Savarirayan, S. ; David, C. S. / Expression of I-A(α)(k) and allelic restriction on A(α)/A(β) pairing in transgenic mice. In: Journal of Immunogenetics. 1990 ; Vol. 17, No. 1-2. pp. 151-159.
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