Expression of HLA-B27 in transgenic mice is dependent on the mouse H-2D genes

C. L. Nickerson, J. Hanson, C. S. David

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Abstract

HLA-B27 transgenic mice in the context of various H-2 haplotypes were produced. A high expression of the HLA-B27 antigen was observed in mice homozygous for H-2b, H-2(f), H-2(s), H-2(p), H-2(r), and H-2(k) haplotypes. Mice of the H-2(v) haplotype expressed HLA-B27 at an intermediate level. Expression of HLA-B27 was minimal in mice of the H-2(q) and H-2(d) haplotypes. This was observed both on the B10 background and in DBA/2 or BALB/c mice. Only minimal expression of HLA-B27 could be detected in B10.PL (K(u)D(d)) or B10.RKDB (K(k)S(k)D(d)L(b)) mice, indicating that the low level of HLA-B27 expression maps to the H-2D gene or a very closely linked gene. Integration and transcription of the HLA-B27 gene does not appear to be different between high-expressing haplotypes as determined by Southern and Northern blot analysis. However, expression of HLA-B27 on the cell surface correlated with the amount of HLA-B27 and β2M that could be immunoprecipitated with an anti-B27 antibody. Therefore, the association of the B27 heavy chain with endogenous β2M and subsequent expression on the cell surface are disrupted in mice with some class I H-2D genes. Possible mechanisms that might contribute to this defect in assembly, transport, and expression of class I molecules are discussed.

Original languageEnglish (US)
Pages (from-to)1255-1261
Number of pages7
JournalJournal of Experimental Medicine
Volume172
Issue number4
StatePublished - 1990

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HLA-B27 Antigen
Transgenic Mice
Haplotypes
Genes
Southern Blotting
Northern Blotting
Anti-Idiotypic Antibodies

ASJC Scopus subject areas

  • Immunology

Cite this

Nickerson, C. L., Hanson, J., & David, C. S. (1990). Expression of HLA-B27 in transgenic mice is dependent on the mouse H-2D genes. Journal of Experimental Medicine, 172(4), 1255-1261.

Expression of HLA-B27 in transgenic mice is dependent on the mouse H-2D genes. / Nickerson, C. L.; Hanson, J.; David, C. S.

In: Journal of Experimental Medicine, Vol. 172, No. 4, 1990, p. 1255-1261.

Research output: Contribution to journalArticle

Nickerson, CL, Hanson, J & David, CS 1990, 'Expression of HLA-B27 in transgenic mice is dependent on the mouse H-2D genes', Journal of Experimental Medicine, vol. 172, no. 4, pp. 1255-1261.
Nickerson, C. L. ; Hanson, J. ; David, C. S. / Expression of HLA-B27 in transgenic mice is dependent on the mouse H-2D genes. In: Journal of Experimental Medicine. 1990 ; Vol. 172, No. 4. pp. 1255-1261.
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