Expression of high- and low-affinity neurotrophin receptors on human transformed B lymphocytes

Angelo Schenone, Jagjit S. Gill, David A. Zacharias, Anthony John Windebank

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

We performed high-sensitivity flow cytometry and Western blotting to study the expression of the low-affinity NGF receptor (p75(NGFR)) and of the transmembrane tyrosine kinase (Trk) family of high-affinity receptors for the different neurotrophic factors on Epstein-Barr virus (EBV)-transformed human B lymphocytes. Reverse transcriptase (RT) polymerase chain reaction (PCR) with single and multiple sets of primers (multiplex RT-PCR) was used to survey the repertoire of neurotrophin receptor transcripts in this cell line. We demonstrated that transformed B cells express detectable levels of Trk b and its mRNA. Conversely, negative results were obtained for p75(NGFR), Trk a, and Trk c. Exposure of EBV-transformed B lymphocytes to brain-derived neurotrophic factor (BDNF) triggered the phosphorylation of Trk b, as demonstrated by Western blots of cell lysates probed with monoclonal antibody against phosphotyrosine.

Original languageEnglish (US)
Pages (from-to)141-149
Number of pages9
JournalJournal of Neuroimmunology
Volume64
Issue number2
DOIs
StatePublished - Feb 1996

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Nerve Growth Factor Receptors
Protein-Tyrosine Kinases
B-Lymphocytes
Nerve Growth Factor Receptor
Reverse Transcriptase Polymerase Chain Reaction
Human Herpesvirus 4
Western Blotting
Phosphotyrosine
Multiplex Polymerase Chain Reaction
Brain-Derived Neurotrophic Factor
Flow Cytometry
Monoclonal Antibodies
Phosphorylation
Cell Line
Messenger RNA

Keywords

  • Brain-derived neurothrophic factor
  • FACS analysis
  • Multiplex RT-PCR
  • Nerve growth factor
  • Neurotrophin-3
  • Neurotrophin-4/5
  • p75(NGFR)
  • Trk a
  • Trk b
  • Trk c
  • Western blotting

ASJC Scopus subject areas

  • Immunology
  • Clinical Neurology
  • Immunology and Allergy
  • Neurology

Cite this

Expression of high- and low-affinity neurotrophin receptors on human transformed B lymphocytes. / Schenone, Angelo; Gill, Jagjit S.; Zacharias, David A.; Windebank, Anthony John.

In: Journal of Neuroimmunology, Vol. 64, No. 2, 02.1996, p. 141-149.

Research output: Contribution to journalArticle

Schenone, Angelo ; Gill, Jagjit S. ; Zacharias, David A. ; Windebank, Anthony John. / Expression of high- and low-affinity neurotrophin receptors on human transformed B lymphocytes. In: Journal of Neuroimmunology. 1996 ; Vol. 64, No. 2. pp. 141-149.
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