Abstract
We performed high-sensitivity flow cytometry and Western blotting to study the expression of the low-affinity NGF receptor (p75(NGFR)) and of the transmembrane tyrosine kinase (Trk) family of high-affinity receptors for the different neurotrophic factors on Epstein-Barr virus (EBV)-transformed human B lymphocytes. Reverse transcriptase (RT) polymerase chain reaction (PCR) with single and multiple sets of primers (multiplex RT-PCR) was used to survey the repertoire of neurotrophin receptor transcripts in this cell line. We demonstrated that transformed B cells express detectable levels of Trk b and its mRNA. Conversely, negative results were obtained for p75(NGFR), Trk a, and Trk c. Exposure of EBV-transformed B lymphocytes to brain-derived neurotrophic factor (BDNF) triggered the phosphorylation of Trk b, as demonstrated by Western blots of cell lysates probed with monoclonal antibody against phosphotyrosine.
Original language | English (US) |
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Pages (from-to) | 141-149 |
Number of pages | 9 |
Journal | Journal of neuroimmunology |
Volume | 64 |
Issue number | 2 |
DOIs | |
State | Published - Feb 1996 |
Keywords
- Brain-derived neurothrophic factor
- FACS analysis
- Multiplex RT-PCR
- Nerve growth factor
- Neurotrophin-3
- Neurotrophin-4/5
- Trk a
- Trk b
- Trk c
- Western blotting
- p75(NGFR)
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology
- Neurology
- Clinical Neurology