Expression of hepatitis B antigens with a simian virus 40 vector

H. Will, R. Cattaneo, E. Pfaff, C. Kuhn, M. Roggendorf, H. Schaller

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

Recombinant DNA molecules consisting of the simian virus 40 (SV40) early region and different subgenomic hepatitis B virus DNA fragments were constructed in vitro and packaged in vivo into SV40 capsids by using a complementing SV40 helper virus. Upon infection with these virus stocks the three known hepatitis B-specific antigens were expressed under SV40 control. The surface antigen was released into the medium, and the core antigen and its derivative hepatitis B e antigen were only detected intracellularly. Size analysis of the core gene product(s) by immunoblotting revealed the presence of a single protein species identical with the 21-kilodalton core antigen isolated from human liver. The hepatitis B core antigen expressing construct did not contain a putative precore sequence, indicating that such a sequence is not needed for hepatitis B core antigen synthesis in animal cells. S1 analysis demonstrated the use of SV40 signals for initiation and polyadenylation of the core gene transcripts. In addition, a processing-polyadenylation signal was identified within the core gene.

Original languageEnglish (US)
Pages (from-to)335-342
Number of pages8
JournalJournal of virology
Volume50
Issue number2
StatePublished - Jun 15 1984

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ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

Will, H., Cattaneo, R., Pfaff, E., Kuhn, C., Roggendorf, M., & Schaller, H. (1984). Expression of hepatitis B antigens with a simian virus 40 vector. Journal of virology, 50(2), 335-342.