Expression of estrogen receptor β messenger RNA variant in breast cancer

Esteban A. Vladusic, Ann E. Hornby, Fabiana K. Guerra-Vladusic, Ruth Lupu

Research output: Contribution to journalArticle

156 Scopus citations

Abstract

Estrogen receptor (ER) β is expressed in a number of tissues, including the breast. We have recently shown that ER-β mRNA is regulated by estradiol (E2) and that antiestrogens antagonize E2 induction of ER-β mRNA. Here, we identify by reverse transcription-PCR and by the RNase protection assay a mRNA coding for a variant of ER-β that is coexpressed with wild-type ER-β in the ER-α-negative, estrogen-independent breast cancer cell line MDA-MB- 231 and in malignant breast tumor specimens. In contrast, this variant was not seen in the tested normal breast tissue. Sequence analysis of the ER-β variant PCR product revealed the absence of 139 bp within the hormone- binding domain. This ER-β deletion corresponds precisely to the entire exon 5 of ER-α. The ER-β variant protein is predicted to lack part of the hormone-binding domain and may bind E2 with lower affinity than the wild- type ER-β protein.

Original languageEnglish (US)
Pages (from-to)210-214
Number of pages5
JournalCancer research
Volume58
Issue number2
StatePublished - Jan 15 1998

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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  • Cite this

    Vladusic, E. A., Hornby, A. E., Guerra-Vladusic, F. K., & Lupu, R. (1998). Expression of estrogen receptor β messenger RNA variant in breast cancer. Cancer research, 58(2), 210-214.