Expression of early growth response genes in human prostate cancer

Manal A. Eid, M. Vijay Kumar, Kenneth A. Iczkowski, David G. Bostwick, Donald J. Tindall

Research output: Contribution to journalArticle

162 Citations (Scopus)

Abstract

Early growth-response (EGR) genes are nuclear transcription factors that are implicated in regulating cell proliferation. Because these genes show divergent expression in various human tumors, we sought to determine their expression in nonmalignant and malignant prostate tissues. Total RNA extracted from prostate tissues was probed with EGR-1, EGR-2, and EGR-α cDNA for Northern blots and digoxigenin-labeled cRNA for in situ hybridization. Both Northern blot and in situ hybridization analyses demonstrated increased EGR-1, but not EGR-2 or EGR-α expression, in malignant prostate tissue as compared with weak expression in nonmalignant tissue. EGR-1 mRNA was quantified in 96 prostate specimens (86 adenocarcinomas representing different Gleason scores and 10 benign tissues showing no histological manifestation of benign prostatic hypertrophy) using in situ hybridization with an 35S-labeled cRNA probe. EGR-1 mRNA was expressed at significantly higher levels in cancer than in normal prostate (P < 0.001). In cancer with Gleason scores 8-10, the expression of EGR-1 was higher compared with those of lower Gleason scores (P < 0.005). Immunohistochemical staining showed predominately basal cell nuclear EGR-1 protein in prostatic acini. Nuclear staining was weak in nonmalignant tissues, more intense in moderately differentiated carcinoma, and most intense in poorly differentiated carcinoma. These results show that EGR-1 is overexpressed in prostate cancer and suggest a role for EGR-1 in prostate cancer growth.

Original languageEnglish (US)
Pages (from-to)2461-2468
Number of pages8
JournalCancer Research
Volume58
Issue number11
StatePublished - Jun 1 1998

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Prostatic Neoplasms
Growth
Genes
Prostate
Neoplasm Grading
In Situ Hybridization
Complementary RNA
Northern Blotting
Early Growth Response Protein 1
Staining and Labeling
Carcinoma
Digoxigenin
Neoplasms
Messenger RNA
Prostatic Hyperplasia
Adenocarcinoma
Transcription Factors
Complementary DNA
Cell Proliferation
RNA

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Eid, M. A., Kumar, M. V., Iczkowski, K. A., Bostwick, D. G., & Tindall, D. J. (1998). Expression of early growth response genes in human prostate cancer. Cancer Research, 58(11), 2461-2468.

Expression of early growth response genes in human prostate cancer. / Eid, Manal A.; Kumar, M. Vijay; Iczkowski, Kenneth A.; Bostwick, David G.; Tindall, Donald J.

In: Cancer Research, Vol. 58, No. 11, 01.06.1998, p. 2461-2468.

Research output: Contribution to journalArticle

Eid, MA, Kumar, MV, Iczkowski, KA, Bostwick, DG & Tindall, DJ 1998, 'Expression of early growth response genes in human prostate cancer', Cancer Research, vol. 58, no. 11, pp. 2461-2468.
Eid MA, Kumar MV, Iczkowski KA, Bostwick DG, Tindall DJ. Expression of early growth response genes in human prostate cancer. Cancer Research. 1998 Jun 1;58(11):2461-2468.
Eid, Manal A. ; Kumar, M. Vijay ; Iczkowski, Kenneth A. ; Bostwick, David G. ; Tindall, Donald J. / Expression of early growth response genes in human prostate cancer. In: Cancer Research. 1998 ; Vol. 58, No. 11. pp. 2461-2468.
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