Expression of Bright at two distinct stages of B lymphocyte development

Carol F. Webb, Elizabeth A. Smith, Kay L. Medina, Kent L. Buchanan, Glennda Smithson, Shenshen Dou

Research output: Contribution to journalArticle

41 Scopus citations

Abstract

The B cell regulator of Ig heavy chain transcription (Bright) is a DNA- binding protein that was originally discovered in a mature Ag-specific B cell line after stimulation with IL-5 and Ag. It binds to the intronic heavy chain enhancer and 5' of the V1 S107 family V(H) promoter. Several studies suggested that Bright may increase transcription of the heavy locus, and expression in cell lines was limited to those representing mature B cells. We have now analyzed normal hemopoietic tissues for the expression of Bright during B lymphocyte differentiation. We expected to find Bright expression in a subset of mature spleen cells, but also observed Bright in a subset of normal B lymphocytic progenitors in both adult bone marrow (BM) and in fetal liver as early as day 12 of gestation. Bright was also expressed in the small percentage of CD4(low) cells in the thymus that are newly arrived from the BM and are not yet committed to the T lymphocyte lineage, but was not observed at later stages of T cell differentiation in either the spleen or thymus. Bright mRNA was not detected in the immature B lymphocytes that initial populate the spleen after migration from the BM. In addition, new splice variants of Bright were observed in fetal tissues. Thus, Bright expression is highly regulated in normal murine lymphocytes and occurs both early and late during B cell differentiation. These findings may have important implications for the function of Bright in regulating Ig transcription.

Original languageEnglish (US)
Pages (from-to)4747-4754
Number of pages8
JournalJournal of Immunology
Volume160
Issue number10
StatePublished - May 15 1998

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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    Webb, C. F., Smith, E. A., Medina, K. L., Buchanan, K. L., Smithson, G., & Dou, S. (1998). Expression of Bright at two distinct stages of B lymphocyte development. Journal of Immunology, 160(10), 4747-4754.