Expression of an immediate early gene, IEX-1, in human tissues

Kathrin A. Feldmann, Mark R. Pittelkow, Patrick C. Roche, Rajiv Kumar, Joseph Peter Grande

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

IEX-1 is an immediate early gene that is induced by ionizing radiation, ultraviolet radiation, and a variety of growth factors. It plays an important role in the regulation of cellular growth. Earlier, we performed studies on the distribution of IEX-1 messenger RNA in different tissues and on the subcellular localization of IEX-1 protein. No reports, however, have appeared concerning the distribution of IEX-1 protein in a variety of human tissues. We raised a polyclonal antibody against a synthetic IEX-1 peptide (amino acids 51-75) and used the antibody to study the distribution of the protein in human tissues. We demonstrate that IEX-1 is strongly expressed in epithelia of the skin, trachea, gastrointestinal, and genitourinary systems, as well as in the pancreas and breast. Endothelial cells within the vasculature of most tissue/organs also strongly express IEX-1. Liver, lung, lymph nodes, and placenta stain weakly. No IEX-1 epitopes were detected in the thymus, testes, ovary, myocardium, skeletal muscle, or spleen. We conclude that IEX-1 is widely expressed in epithelial and endocrine tissues, as well as in vascular endothelium.

Original languageEnglish (US)
Pages (from-to)489-497
Number of pages9
JournalHistochemistry and Cell Biology
Volume115
Issue number6
StatePublished - 2001

Fingerprint

Immediate-Early Genes
genes
Genes
Tissue
proteins
Proteins
antibodies
Epithelium
Antibodies
genitourinary system
gastrointestinal system
Urogenital System
trachea
Vascular Endothelium
ribonucleic acids
endothelium
Epitopes
testes
myocardium
Thymus

Keywords

  • Epithelium
  • IEX-1
  • Immediate early gene
  • Immunohistochemistry
  • Tissue expression

ASJC Scopus subject areas

  • Cell Biology
  • Instrumentation

Cite this

Expression of an immediate early gene, IEX-1, in human tissues. / Feldmann, Kathrin A.; Pittelkow, Mark R.; Roche, Patrick C.; Kumar, Rajiv; Grande, Joseph Peter.

In: Histochemistry and Cell Biology, Vol. 115, No. 6, 2001, p. 489-497.

Research output: Contribution to journalArticle

Feldmann, KA, Pittelkow, MR, Roche, PC, Kumar, R & Grande, JP 2001, 'Expression of an immediate early gene, IEX-1, in human tissues', Histochemistry and Cell Biology, vol. 115, no. 6, pp. 489-497.
Feldmann, Kathrin A. ; Pittelkow, Mark R. ; Roche, Patrick C. ; Kumar, Rajiv ; Grande, Joseph Peter. / Expression of an immediate early gene, IEX-1, in human tissues. In: Histochemistry and Cell Biology. 2001 ; Vol. 115, No. 6. pp. 489-497.
@article{9e7fd57888d844da8c037e543b2e62e0,
title = "Expression of an immediate early gene, IEX-1, in human tissues",
abstract = "IEX-1 is an immediate early gene that is induced by ionizing radiation, ultraviolet radiation, and a variety of growth factors. It plays an important role in the regulation of cellular growth. Earlier, we performed studies on the distribution of IEX-1 messenger RNA in different tissues and on the subcellular localization of IEX-1 protein. No reports, however, have appeared concerning the distribution of IEX-1 protein in a variety of human tissues. We raised a polyclonal antibody against a synthetic IEX-1 peptide (amino acids 51-75) and used the antibody to study the distribution of the protein in human tissues. We demonstrate that IEX-1 is strongly expressed in epithelia of the skin, trachea, gastrointestinal, and genitourinary systems, as well as in the pancreas and breast. Endothelial cells within the vasculature of most tissue/organs also strongly express IEX-1. Liver, lung, lymph nodes, and placenta stain weakly. No IEX-1 epitopes were detected in the thymus, testes, ovary, myocardium, skeletal muscle, or spleen. We conclude that IEX-1 is widely expressed in epithelial and endocrine tissues, as well as in vascular endothelium.",
keywords = "Epithelium, IEX-1, Immediate early gene, Immunohistochemistry, Tissue expression",
author = "Feldmann, {Kathrin A.} and Pittelkow, {Mark R.} and Roche, {Patrick C.} and Rajiv Kumar and Grande, {Joseph Peter}",
year = "2001",
language = "English (US)",
volume = "115",
pages = "489--497",
journal = "Histochemistry and Cell Biology",
issn = "0948-6143",
publisher = "Springer Verlag",
number = "6",

}

TY - JOUR

T1 - Expression of an immediate early gene, IEX-1, in human tissues

AU - Feldmann, Kathrin A.

AU - Pittelkow, Mark R.

AU - Roche, Patrick C.

AU - Kumar, Rajiv

AU - Grande, Joseph Peter

PY - 2001

Y1 - 2001

N2 - IEX-1 is an immediate early gene that is induced by ionizing radiation, ultraviolet radiation, and a variety of growth factors. It plays an important role in the regulation of cellular growth. Earlier, we performed studies on the distribution of IEX-1 messenger RNA in different tissues and on the subcellular localization of IEX-1 protein. No reports, however, have appeared concerning the distribution of IEX-1 protein in a variety of human tissues. We raised a polyclonal antibody against a synthetic IEX-1 peptide (amino acids 51-75) and used the antibody to study the distribution of the protein in human tissues. We demonstrate that IEX-1 is strongly expressed in epithelia of the skin, trachea, gastrointestinal, and genitourinary systems, as well as in the pancreas and breast. Endothelial cells within the vasculature of most tissue/organs also strongly express IEX-1. Liver, lung, lymph nodes, and placenta stain weakly. No IEX-1 epitopes were detected in the thymus, testes, ovary, myocardium, skeletal muscle, or spleen. We conclude that IEX-1 is widely expressed in epithelial and endocrine tissues, as well as in vascular endothelium.

AB - IEX-1 is an immediate early gene that is induced by ionizing radiation, ultraviolet radiation, and a variety of growth factors. It plays an important role in the regulation of cellular growth. Earlier, we performed studies on the distribution of IEX-1 messenger RNA in different tissues and on the subcellular localization of IEX-1 protein. No reports, however, have appeared concerning the distribution of IEX-1 protein in a variety of human tissues. We raised a polyclonal antibody against a synthetic IEX-1 peptide (amino acids 51-75) and used the antibody to study the distribution of the protein in human tissues. We demonstrate that IEX-1 is strongly expressed in epithelia of the skin, trachea, gastrointestinal, and genitourinary systems, as well as in the pancreas and breast. Endothelial cells within the vasculature of most tissue/organs also strongly express IEX-1. Liver, lung, lymph nodes, and placenta stain weakly. No IEX-1 epitopes were detected in the thymus, testes, ovary, myocardium, skeletal muscle, or spleen. We conclude that IEX-1 is widely expressed in epithelial and endocrine tissues, as well as in vascular endothelium.

KW - Epithelium

KW - IEX-1

KW - Immediate early gene

KW - Immunohistochemistry

KW - Tissue expression

UR - http://www.scopus.com/inward/record.url?scp=0034912515&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034912515&partnerID=8YFLogxK

M3 - Article

C2 - 11455449

AN - SCOPUS:0034912515

VL - 115

SP - 489

EP - 497

JO - Histochemistry and Cell Biology

JF - Histochemistry and Cell Biology

SN - 0948-6143

IS - 6

ER -