TY - JOUR
T1 - Expression of an immediate early gene, IEX-1, in human tissues
AU - Feldmann, Kathrin A.
AU - Pittelkow, Mark R.
AU - Roche, Patrick C.
AU - Kumar, Rajiv
AU - Grande, Joseph P.
N1 - Funding Information:
Acknowledgements This work was supported by NIH grants DK 25409 and AR 27032 to R. Kumar and the Mayo Foundation.
PY - 2001
Y1 - 2001
N2 - IEX-1 is an immediate early gene that is induced by ionizing radiation, ultraviolet radiation, and a variety of growth factors. It plays an important role in the regulation of cellular growth. Earlier, we performed studies on the distribution of IEX-1 messenger RNA in different tissues and on the subcellular localization of IEX-1 protein. No reports, however, have appeared concerning the distribution of IEX-1 protein in a variety of human tissues. We raised a polyclonal antibody against a synthetic IEX-1 peptide (amino acids 51-75) and used the antibody to study the distribution of the protein in human tissues. We demonstrate that IEX-1 is strongly expressed in epithelia of the skin, trachea, gastrointestinal, and genitourinary systems, as well as in the pancreas and breast. Endothelial cells within the vasculature of most tissue/organs also strongly express IEX-1. Liver, lung, lymph nodes, and placenta stain weakly. No IEX-1 epitopes were detected in the thymus, testes, ovary, myocardium, skeletal muscle, or spleen. We conclude that IEX-1 is widely expressed in epithelial and endocrine tissues, as well as in vascular endothelium.
AB - IEX-1 is an immediate early gene that is induced by ionizing radiation, ultraviolet radiation, and a variety of growth factors. It plays an important role in the regulation of cellular growth. Earlier, we performed studies on the distribution of IEX-1 messenger RNA in different tissues and on the subcellular localization of IEX-1 protein. No reports, however, have appeared concerning the distribution of IEX-1 protein in a variety of human tissues. We raised a polyclonal antibody against a synthetic IEX-1 peptide (amino acids 51-75) and used the antibody to study the distribution of the protein in human tissues. We demonstrate that IEX-1 is strongly expressed in epithelia of the skin, trachea, gastrointestinal, and genitourinary systems, as well as in the pancreas and breast. Endothelial cells within the vasculature of most tissue/organs also strongly express IEX-1. Liver, lung, lymph nodes, and placenta stain weakly. No IEX-1 epitopes were detected in the thymus, testes, ovary, myocardium, skeletal muscle, or spleen. We conclude that IEX-1 is widely expressed in epithelial and endocrine tissues, as well as in vascular endothelium.
KW - Epithelium
KW - IEX-1
KW - Immediate early gene
KW - Immunohistochemistry
KW - Tissue expression
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U2 - 10.1007/s004180100284
DO - 10.1007/s004180100284
M3 - Article
C2 - 11455449
AN - SCOPUS:0034912515
SN - 0948-6143
VL - 115
SP - 489
EP - 497
JO - Histochemistry and Cell Biology
JF - Histochemistry and Cell Biology
IS - 6
ER -