TY - JOUR
T1 - Expression of an antigen homologous to the human CO17-1A/GA733 colon cancer antigen in animal tissues
AU - Zaloudik, J.
AU - Basak, S.
AU - Nesbit, M.
AU - Speicher, D. W.
AU - Wunner, W. H.
AU - Miller, E.
AU - Ernst-Grotkowski, C.
AU - Kennedy, R.
AU - Bergsagel, L. P.
AU - Koido, T.
AU - Herlyn, D.
N1 - Funding Information:
This work was supported in part by National Institutes of Health Grants CA 605595, CA 10815 and CA 43735, and a grant from Ajinomoto, Japan. We thank Dr Michael Kuehl (NCI, Bethesda, MD, USA) for helpful discussions; Elizabeth Freeman and Dr John Daly (Hospital of the University of Pennsylvania, Philadelphia, PA, USA) for providing human colonic tissue; Marie Prewett, Dawn Marchadier, Elsa Aglow and Margaret Enverso for excellent technical assistance; Joyce Macauley for typing; and Marina Hoffman for editing this manuscript.
PY - 1997
Y1 - 1997
N2 - The CO17-1A/GA733 antigen is associated with human carcinomas and some normal epithelial tissues. This antigen has shown promise as a target in approaches to passive and active immunotherapy of colorectal cancer. The relevance of animal models for studies of immunotherapy targeting this antigen in patients is dependent on the expression of the antigen on normal animal tissues. Immunohistoperoxidase staining with polyclonal rabbit antibodies to the human antigen revealed the human homologue on normal small intestine, colon and liver of mice, rats and non-human primates, whereas mouse monoclonal antibodies to the CO17-1A or GA733 epitopes on the human antigen did not detect the antigen. Polyclonal rabbit antibodies, elicited by the murine antigen homologue derived from recombinant baculovirus-infected insect cells, immunoprecipitated the antigen from mouse small intestine, colon, stomach, kidney and lung. The isolated recombinant murine protein bound polyclonal, but not monoclonal, antibodies to the human CO17-1A/GA733 antigen, and recombinant human antigen bound polyclonal antibodies elicited by the murine antigen homologue. Thus, the antigen homologue expressed by animal tissues is similar, but not identical, to the human antigen. These results have important implications for experimental active and passive immunotherapy targeting the CO17-1A/GA733 antigen.
AB - The CO17-1A/GA733 antigen is associated with human carcinomas and some normal epithelial tissues. This antigen has shown promise as a target in approaches to passive and active immunotherapy of colorectal cancer. The relevance of animal models for studies of immunotherapy targeting this antigen in patients is dependent on the expression of the antigen on normal animal tissues. Immunohistoperoxidase staining with polyclonal rabbit antibodies to the human antigen revealed the human homologue on normal small intestine, colon and liver of mice, rats and non-human primates, whereas mouse monoclonal antibodies to the CO17-1A or GA733 epitopes on the human antigen did not detect the antigen. Polyclonal rabbit antibodies, elicited by the murine antigen homologue derived from recombinant baculovirus-infected insect cells, immunoprecipitated the antigen from mouse small intestine, colon, stomach, kidney and lung. The isolated recombinant murine protein bound polyclonal, but not monoclonal, antibodies to the human CO17-1A/GA733 antigen, and recombinant human antigen bound polyclonal antibodies elicited by the murine antigen homologue. Thus, the antigen homologue expressed by animal tissues is similar, but not identical, to the human antigen. These results have important implications for experimental active and passive immunotherapy targeting the CO17-1A/GA733 antigen.
KW - CO17-1A/GA733 antigen homologue
KW - Colorectal carcinoma
KW - Experimental animals
KW - Murine epithelial glycoprotein
UR - http://www.scopus.com/inward/record.url?scp=9844242130&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=9844242130&partnerID=8YFLogxK
U2 - 10.1038/bjc.1997.483
DO - 10.1038/bjc.1997.483
M3 - Article
C2 - 9328151
AN - SCOPUS:9844242130
SN - 0007-0920
VL - 76
SP - 909
EP - 916
JO - British journal of cancer
JF - British journal of cancer
IS - 7
ER -