Expression of an antigen homologous to the human CO17-1A/GA733 colon cancer antigen in animal tissues

J. Zaloudik, S. Basak, M. Nesbit, D. W. Speicher, W. H. Wunner, E. Miller, C. Ernst-Grotkowski, R. Kennedy, L. P. Bergsagel, T. Koido, D. Herlyn

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

The CO17-1A/GA733 antigen is associated with human carcinomas and some normal epithelial tissues. This antigen has shown promise as a target in approaches to passive and active immunotherapy of colorectal cancer. The relevance of animal models for studies of immunotherapy targeting this antigen in patients is dependent on the expression of the antigen on normal animal tissues. Immunohistoperoxidase staining with polyclonal rabbit antibodies to the human antigen revealed the human homologue on normal small intestine, colon and liver of mice, rats and non-human primates, whereas mouse monoclonal antibodies to the CO17-1A or GA733 epitopes on the human antigen did not detect the antigen. Polyclonal rabbit antibodies, elicited by the murine antigen homologue derived from recombinant baculovirus-infected insect cells, immunoprecipitated the antigen from mouse small intestine, colon, stomach, kidney and lung. The isolated recombinant murine protein bound polyclonal, but not monoclonal, antibodies to the human CO17-1A/GA733 antigen, and recombinant human antigen bound polyclonal antibodies elicited by the murine antigen homologue. Thus, the antigen homologue expressed by animal tissues is similar, but not identical, to the human antigen. These results have important implications for experimental active and passive immunotherapy targeting the CO17-1A/GA733 antigen.

Original languageEnglish (US)
Pages (from-to)909-916
Number of pages8
JournalBritish journal of cancer
Volume76
Issue number7
DOIs
StatePublished - 1997

Keywords

  • CO17-1A/GA733 antigen homologue
  • Colorectal carcinoma
  • Experimental animals
  • Murine epithelial glycoprotein

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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