Expression and transport of Angiotensin II AT1 receptors in spinal cord, dorsal root ganglia and sciatic nerve of the rat

Jaroslav Pavel, Hui Tang, William Stephen Brimijoin, Armen Moughamian, Tsuyoshi Nishioku, Julius Benicky, Juan M. Saavedra

Research output: Contribution to journalArticle

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Abstract

To clarify the role of Angiotensin II in the regulation of peripheral sensory and motor systems, we initiated a study of the expression, localization and transport of Angiotensin II receptor types in the rat sciatic nerve pathway, including L4-L5 spinal cord segments, the corresponding dorsal root ganglia (DRGs) and the sciatic nerve. We used quantitative autoradiography for AT1 and AT2 receptors, and in situ hybridization to detect AT1A, AT1B and AT2 mRNAs. We found substantial expression and discrete localization of Angiotensin II AT1 receptors, with much higher numbers in the grey than in the white matter. A very high AT1 receptor expression was detected in the superficial dorsal horns and in neuronal clusters of the DRGs. Expression of AT1A mRNA was significantly higher than that of AT1B. AT1 receptor binding and AT1A and AT1B mRNAs were especially prominent in ventral horn motor neurons, and in the DRG neuronal cells. Unilateral dorsal rhizotomy significantly reduced AT1 receptor binding in the ipsilateral side of the superficial dorsal horn, indicating that a substantial number of dorsal horn AT1 receptors have their origin in the DRGs. After ligation of the sciatic nerve, there was a high accumulation of AT1 receptors proximal to the ligature, a demonstration of anterograde receptor transport. We found inconsistent levels of AT2 receptor binding and mRNA. Our results suggest multiple roles of Angiotensin II AT1 receptors in the regulation of sensory and motor functions.

Original languageEnglish (US)
Pages (from-to)111-122
Number of pages12
JournalBrain Research
Volume1246
DOIs
StatePublished - Dec 30 2008

Fingerprint

Angiotensin Type 1 Receptor
Angiotensin Receptors
Spinal Ganglia
Sciatic Nerve
Spinal Cord
Messenger RNA
Ligation
Rhizotomy
Anterior Horn Cells
Motor Neurons
Autoradiography
Angiotensin II
In Situ Hybridization
Spinal Cord Dorsal Horn

Keywords

  • Angiotensin II AT and AT receptor types
  • Dorsal rhizotomy.
  • Dorsal root ganglia
  • Renin angiotensin system
  • Sciatic nerve ligation
  • Spinal cord

ASJC Scopus subject areas

  • Neuroscience(all)
  • Clinical Neurology
  • Developmental Biology
  • Molecular Biology

Cite this

Expression and transport of Angiotensin II AT1 receptors in spinal cord, dorsal root ganglia and sciatic nerve of the rat. / Pavel, Jaroslav; Tang, Hui; Brimijoin, William Stephen; Moughamian, Armen; Nishioku, Tsuyoshi; Benicky, Julius; Saavedra, Juan M.

In: Brain Research, Vol. 1246, 30.12.2008, p. 111-122.

Research output: Contribution to journalArticle

Pavel, Jaroslav ; Tang, Hui ; Brimijoin, William Stephen ; Moughamian, Armen ; Nishioku, Tsuyoshi ; Benicky, Julius ; Saavedra, Juan M. / Expression and transport of Angiotensin II AT1 receptors in spinal cord, dorsal root ganglia and sciatic nerve of the rat. In: Brain Research. 2008 ; Vol. 1246. pp. 111-122.
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