Expression and regulatory role of GAIP-interacting protein GIPC in pancreatic adenocarcinoma

Michael H. Muders, Shamit K. Dutta, Ling Wang, Julie S. Lau, Resham Bhattacharya, Thomas Christopher Smyrk, Suresh T Chari, Kaustubh Datta, Debabrata Mukhopadhyay

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Regulator of G-protein signaling-GAIP-interacting protein COOH terminus (GIPC) is involved in protein trafficking, endocytosis, and receptor clustering and is associated with insulin-like growth factor I receptor (IGF-IR), a receptor important for proliferation and anchorage-independent growth. Here, we described GIPC expression in different human pancreatic adenocarcinoma (PCA) cell lines and we examined the role of GIPC in the regulation of IGF-IR protein levels in PCA. Interestingly, inhibition of GIPC expression by RNA interference led to reduced IGF-IR protein levels and a subsequent decrease in proliferation of PCA cells. We also determined that the PDZ domain of GIPC is essential for the post-translational regulation and the binding of IGF-IR. The importance of GIPC in pancreatic cancer development and progression is supported by tissue microarray data of 300 pancreatic cancer specimens where GIPC is highly expressed in PCA. Taken together, our data suggest that GIPC is a central molecule for the stability of IGF-IR and could be a target for future therapy.

Original languageEnglish (US)
Pages (from-to)10264-10268
Number of pages5
JournalCancer Research
Volume66
Issue number21
DOIs
StatePublished - Nov 1 2006

Fingerprint

Adenocarcinoma
IGF Type 1 Receptor
Proteins
Pancreatic Neoplasms
GTP-Binding Protein Regulators
PDZ Domains
Protein Transport
RNA Interference
Endocytosis
Cluster Analysis
Cell Line
Growth

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Expression and regulatory role of GAIP-interacting protein GIPC in pancreatic adenocarcinoma. / Muders, Michael H.; Dutta, Shamit K.; Wang, Ling; Lau, Julie S.; Bhattacharya, Resham; Smyrk, Thomas Christopher; Chari, Suresh T; Datta, Kaustubh; Mukhopadhyay, Debabrata.

In: Cancer Research, Vol. 66, No. 21, 01.11.2006, p. 10264-10268.

Research output: Contribution to journalArticle

Muders, MH, Dutta, SK, Wang, L, Lau, JS, Bhattacharya, R, Smyrk, TC, Chari, ST, Datta, K & Mukhopadhyay, D 2006, 'Expression and regulatory role of GAIP-interacting protein GIPC in pancreatic adenocarcinoma', Cancer Research, vol. 66, no. 21, pp. 10264-10268. https://doi.org/10.1158/0008-5472.CAN-06-2321
Muders MH, Dutta SK, Wang L, Lau JS, Bhattacharya R, Smyrk TC et al. Expression and regulatory role of GAIP-interacting protein GIPC in pancreatic adenocarcinoma. Cancer Research. 2006 Nov 1;66(21):10264-10268. https://doi.org/10.1158/0008-5472.CAN-06-2321
Muders, Michael H. ; Dutta, Shamit K. ; Wang, Ling ; Lau, Julie S. ; Bhattacharya, Resham ; Smyrk, Thomas Christopher ; Chari, Suresh T ; Datta, Kaustubh ; Mukhopadhyay, Debabrata. / Expression and regulatory role of GAIP-interacting protein GIPC in pancreatic adenocarcinoma. In: Cancer Research. 2006 ; Vol. 66, No. 21. pp. 10264-10268.
@article{65bf418b88074951b86967eea4b80f30,
title = "Expression and regulatory role of GAIP-interacting protein GIPC in pancreatic adenocarcinoma",
abstract = "Regulator of G-protein signaling-GAIP-interacting protein COOH terminus (GIPC) is involved in protein trafficking, endocytosis, and receptor clustering and is associated with insulin-like growth factor I receptor (IGF-IR), a receptor important for proliferation and anchorage-independent growth. Here, we described GIPC expression in different human pancreatic adenocarcinoma (PCA) cell lines and we examined the role of GIPC in the regulation of IGF-IR protein levels in PCA. Interestingly, inhibition of GIPC expression by RNA interference led to reduced IGF-IR protein levels and a subsequent decrease in proliferation of PCA cells. We also determined that the PDZ domain of GIPC is essential for the post-translational regulation and the binding of IGF-IR. The importance of GIPC in pancreatic cancer development and progression is supported by tissue microarray data of 300 pancreatic cancer specimens where GIPC is highly expressed in PCA. Taken together, our data suggest that GIPC is a central molecule for the stability of IGF-IR and could be a target for future therapy.",
author = "Muders, {Michael H.} and Dutta, {Shamit K.} and Ling Wang and Lau, {Julie S.} and Resham Bhattacharya and Smyrk, {Thomas Christopher} and Chari, {Suresh T} and Kaustubh Datta and Debabrata Mukhopadhyay",
year = "2006",
month = "11",
day = "1",
doi = "10.1158/0008-5472.CAN-06-2321",
language = "English (US)",
volume = "66",
pages = "10264--10268",
journal = "Journal of Cancer Research",
issn = "0099-7013",
publisher = "American Association for Cancer Research Inc.",
number = "21",

}

TY - JOUR

T1 - Expression and regulatory role of GAIP-interacting protein GIPC in pancreatic adenocarcinoma

AU - Muders, Michael H.

AU - Dutta, Shamit K.

AU - Wang, Ling

AU - Lau, Julie S.

AU - Bhattacharya, Resham

AU - Smyrk, Thomas Christopher

AU - Chari, Suresh T

AU - Datta, Kaustubh

AU - Mukhopadhyay, Debabrata

PY - 2006/11/1

Y1 - 2006/11/1

N2 - Regulator of G-protein signaling-GAIP-interacting protein COOH terminus (GIPC) is involved in protein trafficking, endocytosis, and receptor clustering and is associated with insulin-like growth factor I receptor (IGF-IR), a receptor important for proliferation and anchorage-independent growth. Here, we described GIPC expression in different human pancreatic adenocarcinoma (PCA) cell lines and we examined the role of GIPC in the regulation of IGF-IR protein levels in PCA. Interestingly, inhibition of GIPC expression by RNA interference led to reduced IGF-IR protein levels and a subsequent decrease in proliferation of PCA cells. We also determined that the PDZ domain of GIPC is essential for the post-translational regulation and the binding of IGF-IR. The importance of GIPC in pancreatic cancer development and progression is supported by tissue microarray data of 300 pancreatic cancer specimens where GIPC is highly expressed in PCA. Taken together, our data suggest that GIPC is a central molecule for the stability of IGF-IR and could be a target for future therapy.

AB - Regulator of G-protein signaling-GAIP-interacting protein COOH terminus (GIPC) is involved in protein trafficking, endocytosis, and receptor clustering and is associated with insulin-like growth factor I receptor (IGF-IR), a receptor important for proliferation and anchorage-independent growth. Here, we described GIPC expression in different human pancreatic adenocarcinoma (PCA) cell lines and we examined the role of GIPC in the regulation of IGF-IR protein levels in PCA. Interestingly, inhibition of GIPC expression by RNA interference led to reduced IGF-IR protein levels and a subsequent decrease in proliferation of PCA cells. We also determined that the PDZ domain of GIPC is essential for the post-translational regulation and the binding of IGF-IR. The importance of GIPC in pancreatic cancer development and progression is supported by tissue microarray data of 300 pancreatic cancer specimens where GIPC is highly expressed in PCA. Taken together, our data suggest that GIPC is a central molecule for the stability of IGF-IR and could be a target for future therapy.

UR - http://www.scopus.com/inward/record.url?scp=33751287963&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33751287963&partnerID=8YFLogxK

U2 - 10.1158/0008-5472.CAN-06-2321

DO - 10.1158/0008-5472.CAN-06-2321

M3 - Article

C2 - 17079444

AN - SCOPUS:33751287963

VL - 66

SP - 10264

EP - 10268

JO - Journal of Cancer Research

JF - Journal of Cancer Research

SN - 0099-7013

IS - 21

ER -