Expression and function of mutant Ia antigen in transgenic mice

B. Y. Wei, J. Martin, R. Little, G. Anderson, S. Savarirayan, J. M. Buerstedde, D. McKean, C. David

Research output: Contribution to journalArticle

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Abstract

Cell surface expression of Ia antigens requires the assembly of α and β heterodimers. We have produced a double transgenic mouse with a wild form A(α)(k) gene and a mutant A(β)(k) (A(β)(k) MB) gene with d-allele substitution at positions 63 and 65-67. Initial studies indicated that the A(α)(k) and A(β)(k) MB transgenes are not expressed on the surface of lymphoid cells of the transgenic mice. However, when spleen cells were stimulated with LPS prior to FACS analyses, A(k)/A(k) MB assembly and subsequent surface expression was induced. The tail skins from transgenic founder mice were rejected by the parental mice indicating a role for the mutant antigen on the allograft. In addition, the A(k) transgenic mice on H-2(q/q) background can partially delete V(β)6+ T cells, suggesting the presence of the transgene product in the thymus.

Original languageEnglish (US)
Pages (from-to)696-701
Number of pages6
JournalTransplantation
Volume50
Issue number4
StatePublished - 1990

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Histocompatibility Antigens Class II
Transgenic Mice
Transgenes
Thymus Gland
Genes
Allografts
Tail
Spleen
Alleles
Lymphocytes
T-Lymphocytes
Antigens
Skin

ASJC Scopus subject areas

  • Immunology
  • Transplantation

Cite this

Wei, B. Y., Martin, J., Little, R., Anderson, G., Savarirayan, S., Buerstedde, J. M., ... David, C. (1990). Expression and function of mutant Ia antigen in transgenic mice. Transplantation, 50(4), 696-701.

Expression and function of mutant Ia antigen in transgenic mice. / Wei, B. Y.; Martin, J.; Little, R.; Anderson, G.; Savarirayan, S.; Buerstedde, J. M.; McKean, D.; David, C.

In: Transplantation, Vol. 50, No. 4, 1990, p. 696-701.

Research output: Contribution to journalArticle

Wei, BY, Martin, J, Little, R, Anderson, G, Savarirayan, S, Buerstedde, JM, McKean, D & David, C 1990, 'Expression and function of mutant Ia antigen in transgenic mice', Transplantation, vol. 50, no. 4, pp. 696-701.
Wei BY, Martin J, Little R, Anderson G, Savarirayan S, Buerstedde JM et al. Expression and function of mutant Ia antigen in transgenic mice. Transplantation. 1990;50(4):696-701.
Wei, B. Y. ; Martin, J. ; Little, R. ; Anderson, G. ; Savarirayan, S. ; Buerstedde, J. M. ; McKean, D. ; David, C. / Expression and function of mutant Ia antigen in transgenic mice. In: Transplantation. 1990 ; Vol. 50, No. 4. pp. 696-701.
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AU - Wei, B. Y.

AU - Martin, J.

AU - Little, R.

AU - Anderson, G.

AU - Savarirayan, S.

AU - Buerstedde, J. M.

AU - McKean, D.

AU - David, C.

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