Expression and function of mutant Ia antigen in transgenic mice

Bing Yuan Wei, Javier Martin, Roger Little, Gary Anderson, Suresh Savarirayan, Jean Marie Buerstedde, David McKean, Chella David

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Cell surface expression of la antigens requires the assembly of α and β heterodimers. We have produced a double transgenic mouse with a wild form gene and a mutant Aβ k (Aβ k MB) gene with d-allele substitution at positions 63 and 65-67. Initial studies indicated that the Aβ k and Aβ k MB transgenes are not expressed on the surface of lymphoid cells of the transgenic mice. However, when spleen cells were stimulated with LPS prior to FACS analyses, Ak/Ak MB assembly and subsequent surface expression was induced. The tail skins from transgenic founder mice were rejected by the parental mice indicating a role for the mutant antigen on the allograft. In addition, the Ak transgenic mice on H-2q/q background can partially delete Vβ6+ T cells, suggesting the presence of the transgene product in the thymus.

Original languageEnglish (US)
Pages (from-to)696-701
Number of pages6
JournalTransplantation
Volume50
Issue number4
DOIs
StatePublished - Oct 1990

ASJC Scopus subject areas

  • Transplantation

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