Cell surface expression of la antigens requires the assembly of α and β heterodimers. We have produced a double transgenic mouse with a wild form gene and a mutant Aβ k (Aβ k MB) gene with d-allele substitution at positions 63 and 65-67. Initial studies indicated that the Aβ k and Aβ k MB transgenes are not expressed on the surface of lymphoid cells of the transgenic mice. However, when spleen cells were stimulated with LPS prior to FACS analyses, Ak/Ak MB assembly and subsequent surface expression was induced. The tail skins from transgenic founder mice were rejected by the parental mice indicating a role for the mutant antigen on the allograft. In addition, the Ak transgenic mice on H-2q/q background can partially delete Vβ6+ T cells, suggesting the presence of the transgene product in the thymus.
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