Expression and function of intestinal hexose transporters after small intestinal denervation

Corey W. Iqbal, Javairiah Fatima, Judith Duenes, Scott G. Houghton, Michael S. Kasparek, Michael G. Sarr

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Background: The role of neural regulation in expression and function of intestinal hexose transporters is unknown. The aim of this study is to determine the role of intestinal innervation in gene expression and function of the membrane hexose transporters, SGLT1, GLUT2, and GLUT5 in the enterocyte. We hypothesize that denervation of the small intestine decreases expression of hexose transporters, which leads to decreased glucose absorption. Methods: Six groups of Lewis rats were studied (n = 6 each) as follows: control, 1 week after sham laparotomy, 1 and 8 weeks after syngeneic (no immune rejection) orthotopic small-bowel transplantation (SBT) (SBT1 and SBT8) to induce complete extrinsic denervation, and 1 and 8 weeks after selective disruption of intrinsic neural continuity to jejunoileum by gut transection and reanastomosis (T/A1 and T/A8). All tissue was harvested between 8 am and 10 am. In duodenum, jejunum, and ileum, mucosal messenger RNA (mRNA) levels were quantitated by real-time polymerase chain reaction (PCR), protein by Western blotting, and transporter-mediated glucose absorption using the everted sleeve technique. Results: Across the 6 groups, the relative gene expression of hexose transporter mRNA and protein levels were unchanged, and no difference in transporter-mediated glucose uptake was evident in any region. The glucose transporter affinity (Km) and functional transporter levels (Vmax) calculated for duodenum and jejunum showed no difference among the 6 groups. Conclusion: Baseline regulation of hexose transporter function is not mediated tonically by intrinsic or extrinsic neural continuity to the jejunoileum.

Original languageEnglish (US)
Pages (from-to)100-112
Number of pages13
JournalSurgery
Volume146
Issue number1
DOIs
StatePublished - Jul 2009

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Monosaccharide Transport Proteins
Denervation
Facilitative Glucose Transport Proteins
Jejunum
Duodenum
Gene Expression
Messenger RNA
Membrane Transport Proteins
Enterocytes
Ileum
Laparotomy
Small Intestine
Real-Time Polymerase Chain Reaction
Proteins
Transplantation
Western Blotting
Glucose

ASJC Scopus subject areas

  • Surgery

Cite this

Iqbal, C. W., Fatima, J., Duenes, J., Houghton, S. G., Kasparek, M. S., & Sarr, M. G. (2009). Expression and function of intestinal hexose transporters after small intestinal denervation. Surgery, 146(1), 100-112. https://doi.org/10.1016/j.surg.2009.02.012

Expression and function of intestinal hexose transporters after small intestinal denervation. / Iqbal, Corey W.; Fatima, Javairiah; Duenes, Judith; Houghton, Scott G.; Kasparek, Michael S.; Sarr, Michael G.

In: Surgery, Vol. 146, No. 1, 07.2009, p. 100-112.

Research output: Contribution to journalArticle

Iqbal, CW, Fatima, J, Duenes, J, Houghton, SG, Kasparek, MS & Sarr, MG 2009, 'Expression and function of intestinal hexose transporters after small intestinal denervation', Surgery, vol. 146, no. 1, pp. 100-112. https://doi.org/10.1016/j.surg.2009.02.012
Iqbal CW, Fatima J, Duenes J, Houghton SG, Kasparek MS, Sarr MG. Expression and function of intestinal hexose transporters after small intestinal denervation. Surgery. 2009 Jul;146(1):100-112. https://doi.org/10.1016/j.surg.2009.02.012
Iqbal, Corey W. ; Fatima, Javairiah ; Duenes, Judith ; Houghton, Scott G. ; Kasparek, Michael S. ; Sarr, Michael G. / Expression and function of intestinal hexose transporters after small intestinal denervation. In: Surgery. 2009 ; Vol. 146, No. 1. pp. 100-112.
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