Expression and characterization of glycophospholipid-anchored human immunodeficiency virus type 1 envelope glycoproteins

Karl Salzwedel, Patrick Bruce Johnston, Susan J. Roberts, John W. Dubay, Eric Hunter

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

Four chimeric human immunodeficiency virus type 1 (HIV-1) env genes were constructed which encoded the extracellular domain of either the wild-type or a cleavage-defective HIV-1 envelope glycoprotein (gp160) fused at one of two different positions in env to a C-terminal glycosyl-phosphatidylinositol (GPI) attachment signal from the mouse Thy-1.1 glycoprotein. All four of the constructs encoded glycoproteins that were efficiently expressed when Rev was supplied in trans, and the two cleavable forms were processed normally to gp120 and a chimeric "gp41." The chimeric glycoproteins, in contrast to the wild-type glycoprotein, could be cleaved from the surface of transfected cells by treatment with phosphatidylinositol-specific phospholipase C, indicating that they were anchored in the plasma membrane by a GPI moiety. These GPI-anchored glycoproteins were transported intracellularly at a rate only slightly lower than that of the full-length HIV-1 glycoprotein and were present on the cell surface in equivalent amounts. Nevertheless, all four glycoproteins were defective in mediating both cell-cell and virus-cell fusion as determined by syncytium formation in COS-1-HeLa-T4 cell mixtures and trans complementation of an env-defective HIV-1 genome.

Original languageEnglish (US)
Pages (from-to)5279-5288
Number of pages10
JournalJournal of Virology
Volume67
Issue number9
StatePublished - Sep 1993
Externally publishedYes

Fingerprint

glycophospholipids
Human immunodeficiency virus 1
HIV-1
glycoproteins
Glycoproteins
phosphatidylinositols
Glycosylphosphatidylinositols
Defective Viruses
CD4-positive T-lymphocytes
cells
CD4-Positive T-Lymphocytes
env Genes
Phosphoinositide Phospholipase C
cell fusion
phospholipase C
Cell Fusion
giant cells
Giant Cells
HeLa Cells
plasma membrane

ASJC Scopus subject areas

  • Immunology

Cite this

Expression and characterization of glycophospholipid-anchored human immunodeficiency virus type 1 envelope glycoproteins. / Salzwedel, Karl; Johnston, Patrick Bruce; Roberts, Susan J.; Dubay, John W.; Hunter, Eric.

In: Journal of Virology, Vol. 67, No. 9, 09.1993, p. 5279-5288.

Research output: Contribution to journalArticle

Salzwedel, Karl ; Johnston, Patrick Bruce ; Roberts, Susan J. ; Dubay, John W. ; Hunter, Eric. / Expression and characterization of glycophospholipid-anchored human immunodeficiency virus type 1 envelope glycoproteins. In: Journal of Virology. 1993 ; Vol. 67, No. 9. pp. 5279-5288.
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