Expression analysis of PAC1-R and PACAP genes in Zebrafish embryos

David Alexandre, Jessy Alonzeau, Brent R. Bill, Stephen C. Ekker, James A. Waschek

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

This study describes the expression of the pituitary adenylate cyclase-activating polypeptide (PACAP1 and PACAP2) and PAC1 receptor genes (PAC1a-R and PAC1b-R) in the brain of zebrafish (Danio rerio) during development. In situ hybridization of the 24- and 48-hpf embryos revealed that PACAP genes were expressed in the telencephalon, the diencephalon, the rhombencephalon, and the neurons in the dorsal part of the spinal cord. PACAP2 mRNA appears to be the most abundant form during brain development. The two PAC1-R subtypes showed a similar expression pattern: mRNAs were detected in the forebrain, the thalamus, and the rhombencephalon. However, in the tectum, only PAC1b-R gene was detected. These results suggest that, in fish, PACAP may play a role in brain development.

Original languageEnglish (US)
Pages (from-to)94-100
Number of pages7
JournalJournal of Molecular Neuroscience
Volume43
Issue number1
DOIs
StatePublished - Jan 1 2011

    Fingerprint

Keywords

  • Central nervous system
  • Ontogeny
  • PACAP receptors
  • Zebrafish

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

Cite this