Experimental validation of an optofluidic platform for microbial single cell isolation and whole genome amplification for human microbiome applications

Yuguang Liu, Patricio Jeraldo, Samantha McDonough, Jin Jen, Robin Patel, Marina Walther-Antonio, Christopher Lambert, Bruce Gale

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Single microbial cell genome sequencing is becoming a powerful tool for the discovery of the hidden genetic information valuable for many medical applications. One of the critical steps in single-cell genome sequencing is the physical isolation of individual cells from a highly diverse heterogeneous population. Amplifying the genome of a single microbial cell is another challenge due to the minute amount of DNA. Efforts have been directed in developing an optofluidic platform integrating advanced microscopy, optical tweezers and microfluidic technology for single cell isolation and genome amplification. Here, we investigate and evaluate the validity of this platform for single microbial cell genome amplification. The successful validation of this approach allows us to perform various single cell studies using this platform.

Original languageEnglish (US)
Title of host publication2017 IEEE International Symposium on Medical Measurements and Applications, MeMeA 2017 - Proceedings
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages62-66
Number of pages5
ISBN (Electronic)9781509029839
DOIs
StatePublished - Jul 19 2017
Event12th IEEE International Symposium on Medical Measurements and Applications, MeMeA 2017 - Rochester, United States
Duration: May 7 2017May 10 2017

Other

Other12th IEEE International Symposium on Medical Measurements and Applications, MeMeA 2017
CountryUnited States
CityRochester
Period5/7/175/10/17

Fingerprint

Cell Separation
genome
Microbiota
Amplification
isolation
platforms
Genes
Genome
Microbial Genome
cells
sequencing
Optical Tweezers
Optical tweezers
Microfluidics
Medical applications
Microscopy
Microscopic examination
DNA
Technology
deoxyribonucleic acid

Keywords

  • microbiome
  • microfluidics
  • optical tweezers
  • optofluidics
  • single cell
  • whole genome amplification

ASJC Scopus subject areas

  • Instrumentation
  • Computer Science Applications
  • Medicine (miscellaneous)

Cite this

Liu, Y., Jeraldo, P., McDonough, S., Jen, J., Patel, R., Walther-Antonio, M., ... Gale, B. (2017). Experimental validation of an optofluidic platform for microbial single cell isolation and whole genome amplification for human microbiome applications. In 2017 IEEE International Symposium on Medical Measurements and Applications, MeMeA 2017 - Proceedings (pp. 62-66). [7985850] Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/MeMeA.2017.7985850

Experimental validation of an optofluidic platform for microbial single cell isolation and whole genome amplification for human microbiome applications. / Liu, Yuguang; Jeraldo, Patricio; McDonough, Samantha; Jen, Jin; Patel, Robin; Walther-Antonio, Marina; Lambert, Christopher; Gale, Bruce.

2017 IEEE International Symposium on Medical Measurements and Applications, MeMeA 2017 - Proceedings. Institute of Electrical and Electronics Engineers Inc., 2017. p. 62-66 7985850.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Liu, Y, Jeraldo, P, McDonough, S, Jen, J, Patel, R, Walther-Antonio, M, Lambert, C & Gale, B 2017, Experimental validation of an optofluidic platform for microbial single cell isolation and whole genome amplification for human microbiome applications. in 2017 IEEE International Symposium on Medical Measurements and Applications, MeMeA 2017 - Proceedings., 7985850, Institute of Electrical and Electronics Engineers Inc., pp. 62-66, 12th IEEE International Symposium on Medical Measurements and Applications, MeMeA 2017, Rochester, United States, 5/7/17. https://doi.org/10.1109/MeMeA.2017.7985850
Liu Y, Jeraldo P, McDonough S, Jen J, Patel R, Walther-Antonio M et al. Experimental validation of an optofluidic platform for microbial single cell isolation and whole genome amplification for human microbiome applications. In 2017 IEEE International Symposium on Medical Measurements and Applications, MeMeA 2017 - Proceedings. Institute of Electrical and Electronics Engineers Inc. 2017. p. 62-66. 7985850 https://doi.org/10.1109/MeMeA.2017.7985850
Liu, Yuguang ; Jeraldo, Patricio ; McDonough, Samantha ; Jen, Jin ; Patel, Robin ; Walther-Antonio, Marina ; Lambert, Christopher ; Gale, Bruce. / Experimental validation of an optofluidic platform for microbial single cell isolation and whole genome amplification for human microbiome applications. 2017 IEEE International Symposium on Medical Measurements and Applications, MeMeA 2017 - Proceedings. Institute of Electrical and Electronics Engineers Inc., 2017. pp. 62-66
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