Experimental Metabolic Syndrome Model Associated with Mechanical and Structural Degenerative Changes of the Aortic Valve

Jason L. Go, Komal Prem, Mohammed A. Al-Hijji, Qing Qin, Christopher Noble, Melissa D Young, Lilach O Lerman, Amir Lerman

Research output: Contribution to journalArticle

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Abstract

The purpose of this study was to test the hypothesis that an experimental high fat (HF) animal with metabolic syndrome results in structural degeneration of the aortic valve. Domestic pigs were divided (n = 12) and administered either a normal or HF diet. After 16-weeks, the HF diet group had increased weight (p ≤ 0.05), total cholesterol (p ≤ 0.05), and systolic and diastolic pressure (p ≤ 0.05). The aortic valve extracellular matrix showed loss of elastin fibers and increased collagen deposition in the HF diet group. Collagen was quantified with ELISA, which showed an increased concentration of collagen types 1 and 3 (p ≤ 0.05). In the HF diet group, the initial stages of microcalcification were observed. Uniaxial mechanical testing of aortic cusps revealed that the HF diet group expressed a decrease in ultimate tensile strength and elastic modulus compared to the control diet group (p ≤ 0.05). Western blot and immunohistochemistry indicated the presence of proteins: lipoprotein-associated phospholipase A2, osteopontin, and osteocalcin with an increased expression in the HF diet group. The current study demonstrates that experimental metabolic syndrome induced by a 16-week HF diet was associated with a statistically significant alteration to the physical architecture of the aortic valve.

Original languageEnglish (US)
Article number17835
JournalScientific Reports
Volume8
Issue number1
DOIs
StatePublished - Dec 1 2018

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High Fat Diet
Aortic Valve
Collagen
1-Alkyl-2-acetylglycerophosphocholine Esterase
Blood Pressure
Calcinosis
Sus scrofa
Osteopontin
Elastin
Tensile Strength
Elastic Modulus
Osteocalcin
Collagen Type I
Extracellular Matrix
Western Blotting
Enzyme-Linked Immunosorbent Assay
Immunohistochemistry
Fats
Cholesterol
Diet

ASJC Scopus subject areas

  • General

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Experimental Metabolic Syndrome Model Associated with Mechanical and Structural Degenerative Changes of the Aortic Valve. / Go, Jason L.; Prem, Komal; Al-Hijji, Mohammed A.; Qin, Qing; Noble, Christopher; Young, Melissa D; Lerman, Lilach O; Lerman, Amir.

In: Scientific Reports, Vol. 8, No. 1, 17835, 01.12.2018.

Research output: Contribution to journalArticle

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