Experimental extrinsic allergic alveolitis and pulmonary angiitis induced by intratracheal or intravenous challenge with Corynebacterium parvum in sensitized rats

Eunhee S. Yi, Hyesun Lee, Yae Kyung Suh, Winson Tang, Meiying Qi, Songmei Yin, Daniel G. Remick, Thomas R. Ulich

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Extrinsic allergic alveolitis and pulmonary sarcoidosis are granulomatous diseases of the lung for which clinical presentation and anatomic site of granuloma formation differ. Extrinsic allergic alveolitis is caused by inhaled antigens, whereas the nature and source of the inciting antigen in sarcoidosis is unknown. To test the hypothesis that the route via which antigen is introduced to the lung contributes to the clinicopathological presentation of pulmonary granulomatous disease, rats immunized with intravenous (i.v.) Corynebacterium parvum were challenged after 2 weeks with either intratracheal (i.t.) or i.v. C. parvum. The granulomatous inflammation elicited by i.t. challenge predominantly involved alveolar spaces and histologically simulated extrinsic allergic alveolitis. In contrast, the inflammation induced by i.v. challenge was characterized by granulomatous angiitis and interstitial inflammation simulating sarcoidosis. Elevations of leukocyte counts and TNF levels in bronchoalveolar fluid, which reflect inflammation tn the intra-alveolar compartment, were much more pronounced after i.t. than after i.v. challenge. Tumor necrosis factor, interleukin-6, CC chemokine, CXC chemokine, and adhesion molecule mRNA and protein expression occurred in each model. In conclusion, i.t. or i.v. challenge with C. parvum in sensitized rats caused pulmonary granulomatous inflammation that was histologically similar to human extrinsic allergic alveolitis and sarcoidosis, respectively. Although the soluble and cellular mediators of granulomatous inflammation were qualitatively similar in both disease models, the differing anatomic source of the same antigenic challenge was responsible for differing clinico-pathological presentations.

Original languageEnglish (US)
Pages (from-to)1303-1312
Number of pages10
JournalAmerican Journal of Pathology
Volume149
Issue number4
StatePublished - Oct 1996
Externally publishedYes

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Extrinsic Allergic Alveolitis
Propionibacterium acnes
Vasculitis
Sarcoidosis
Inflammation
Lung
Antigens
Lung Diseases
Central Nervous System Vasculitis
Pulmonary Sarcoidosis
Anatomic Models
CXC Chemokines
Inflammation Mediators
CC Chemokines
Granuloma
Leukocyte Count
Interleukin-6
Pneumonia
Tumor Necrosis Factor-alpha
Messenger RNA

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Experimental extrinsic allergic alveolitis and pulmonary angiitis induced by intratracheal or intravenous challenge with Corynebacterium parvum in sensitized rats. / Yi, Eunhee S.; Lee, Hyesun; Suh, Yae Kyung; Tang, Winson; Qi, Meiying; Yin, Songmei; Remick, Daniel G.; Ulich, Thomas R.

In: American Journal of Pathology, Vol. 149, No. 4, 10.1996, p. 1303-1312.

Research output: Contribution to journalArticle

Yi, Eunhee S. ; Lee, Hyesun ; Suh, Yae Kyung ; Tang, Winson ; Qi, Meiying ; Yin, Songmei ; Remick, Daniel G. ; Ulich, Thomas R. / Experimental extrinsic allergic alveolitis and pulmonary angiitis induced by intratracheal or intravenous challenge with Corynebacterium parvum in sensitized rats. In: American Journal of Pathology. 1996 ; Vol. 149, No. 4. pp. 1303-1312.
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