Experimental autoimmune myasthenia gravis and myasthenia gravis: biochemical and immunochemical aspects.

J. M. Lindstrom, Vanda A Lennon, M. E. Seybold, S. Whittingham

Research output: Contribution to journalArticle

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Abstract

Stucture of acetylcholine receptor protein (AChR) purified from Electrophorus electricus (eel) by affinity chromatography is described. AChR is detected in extracts from human muscle, rat muscle, and rat thymus. Rats immunized with eel AChR develop humoral antibodies, a small fraction of which recognize AChR from rat muscle. Rats immunized with AChR exhibit myasthenia, but those immunized with denatured AChR do not. Immunoglobulin fraction of antisera to eel AChR can block the activity of AChR in electroplaques. Sera from patients with myasthenia gravis contain antibodies to AChR from human muscle detectabe at an average value 300-fold the background level in sera from nonmyasthenics. Relationship of thymoma and disease intensity to antibody titer is examined. The chronic phase of EAMG appears a good model of MG, since in both cases similar concentrations of 7-S immunoglobulin against determinants on muscle AChR other than the toxin binding site are found. Assay of anti-AChR antibody in sera from MG patients using AChR from rat muscle gives titers 10%-15% of those obtained using AChR from human muscle, and using AChR from eel gives negligible titers. The assay method described for assaying antibodies against AChR from human muscle is suggested as a diagnostic test for MG.

Original languageEnglish (US)
Pages (from-to)254-274
Number of pages21
JournalAnnals of the New York Academy of Sciences
Volume274
StatePublished - 1976
Externally publishedYes

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Autoimmune Experimental Myasthenia Gravis
Myasthenia Gravis
Cholinergic Receptors
Muscle
Proteins
Electrophorus
Muscle Proteins
Rats
Antibodies
Protein
Muscles
Immunoglobulins
Assays
Serum
Affinity chromatography
Thymus
Thymoma

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Experimental autoimmune myasthenia gravis and myasthenia gravis : biochemical and immunochemical aspects. / Lindstrom, J. M.; Lennon, Vanda A; Seybold, M. E.; Whittingham, S.

In: Annals of the New York Academy of Sciences, Vol. 274, 1976, p. 254-274.

Research output: Contribution to journalArticle

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