TY - JOUR
T1 - Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells
AU - Valadi, Hadi
AU - Ekström, Karin
AU - Bossios, Apostolos
AU - Sjöstrand, Margareta
AU - Lee, James J.
AU - Lötvall, Jan O.
N1 - Funding Information:
We gratefully acknowledge Swegene Microarray Resource Centre at Lund University for help with the Affymetrix microarray processing and analysis and the Exiqon Company for analysing the microRNA. We thank core facility, Proteomics Resource Centre at Göteborg University (http://www.proteomics. cf.gu.se) for the help with LC-MS/MS and MALDI-TOF. The human mast cell line HMC-1 was kindly provided by G. Nilsson (Uppsala University) and J. Butterfield (Mayo Clinic). We have had exceedingly helpful discussions with E. Telemo and K. McNagny during the progress of the project. We thank B. R. Johansson, U. Nannmark and Y. Josefsson at The Electron Microscopy Unit, Inst Biomedicine, Göteborg University for the help with electron microscopy, and N. Almqvist for taking several of the electron microscopy photographs. We are also grateful to T. Nyström and A. Farwell for providing laboratory resources for some of the experiments at the Cell and Molecular Biology Laboratory at Göteborg University. The current experiments were funded by the Swedish Research Council (K2005-74X-13429-06A), the Swedish Heart and Lung foundation and the Swedish Asthma-Allergy Foundation. J.L. is funded by Herman Krefting’s Foundation against Asthma/Allergy.
PY - 2007/6
Y1 - 2007/6
N2 - Exosomes are vesicles of endocytic origin released by many cells. These vesicles can mediate communication between cells, facilitating processes such as antigen presentation. Here, we show that exosomes from a mouse and a human mast cell line (MC/9 and HMC-1, respectively), as well as primary bone marrow-derived mouse mast cells, contain RNA. Microarray assessments revealed the presence of mRNA from approximately 1300 genes, many of which are not present in the cytoplasm of the donor cell. In vitro translation proved that the exosome mRNAs were functional. Quality control RNA analysis of total RNA derived from exosomes also revealed presence of small RNAs, including microRNAs. The RNA from mast cell exosomes is transferable to other mouse and human mast cells. After transfer of mouse exosomal RNA to human mast cells, new mouse proteins were found in the recipient cells, indicating that transferred exosomal mRNA can be translated after entering another cell. In summary, we show that exosomes contain both mRNA and microRNA, which can be delivered to another cell, and can be functional in this new location. We propose that this RNA is called "exosomal shuttle RNA" (esRNA).
AB - Exosomes are vesicles of endocytic origin released by many cells. These vesicles can mediate communication between cells, facilitating processes such as antigen presentation. Here, we show that exosomes from a mouse and a human mast cell line (MC/9 and HMC-1, respectively), as well as primary bone marrow-derived mouse mast cells, contain RNA. Microarray assessments revealed the presence of mRNA from approximately 1300 genes, many of which are not present in the cytoplasm of the donor cell. In vitro translation proved that the exosome mRNAs were functional. Quality control RNA analysis of total RNA derived from exosomes also revealed presence of small RNAs, including microRNAs. The RNA from mast cell exosomes is transferable to other mouse and human mast cells. After transfer of mouse exosomal RNA to human mast cells, new mouse proteins were found in the recipient cells, indicating that transferred exosomal mRNA can be translated after entering another cell. In summary, we show that exosomes contain both mRNA and microRNA, which can be delivered to another cell, and can be functional in this new location. We propose that this RNA is called "exosomal shuttle RNA" (esRNA).
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U2 - 10.1038/ncb1596
DO - 10.1038/ncb1596
M3 - Article
C2 - 17486113
AN - SCOPUS:34249302620
SN - 1465-7392
VL - 9
SP - 654
EP - 659
JO - Nature Cell Biology
JF - Nature Cell Biology
IS - 6
ER -