Exogenous spermine reduces ischemic damage in a model of focal cerebral ischemia in the rat

Bert A. Coert, Robert E. Anderson, Fredric B. Meyer

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

Alterations in polyamine metabolism during and after global or focal cerebral ischemia can produce a multiplicity of effects on brain such as modification in mitochondria calcium buffering capacity, exacerbating glutamate-mediated neurotoxicity, and impairment of the blood-brain barrier. In this study, the endogenous polyamine spermine was administered intravenously 30 min prior to temporary focal cerebral ischemia in rats induced by clipping of the left middle cerebral and bilateral common carotid arteries for 3 h. Three days after removal of the microclips, intracardiac perfusion with 2% 2,3,5-triphenyl tetrazolium chloride was performed. Coronal slices were cut, photographed, and examined for cortical infarct volume. Spermine reduced infarct volume in a dose-dependent fashion. This study demonstrates that the use of polyamines may be considered as a powerful tool in prevention of ischemic tissue damage following focal cerebral ischemia. Copyright (C) 2000 Elsevier Science Ireland Ltd.

Original languageEnglish (US)
Pages (from-to)5-8
Number of pages4
JournalNeuroscience Letters
Volume282
Issue number1-2
DOIs
StatePublished - Mar 17 2000

Keywords

  • Infarction volume
  • Neuroprotection
  • Rats
  • Reversible focal cerebral ischemia
  • Spermine

ASJC Scopus subject areas

  • Neuroscience(all)

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