Exogenous mycoplasmal p37 protein alters gene expression, growth and morphology of prostate cancer cells

Steven Goodison, K. Nakamura, K. A. Iczkowski, S. Anai, S. K. Boehlein, C. J. Rosser

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

We previously showed that the Mycoplasma hyorhinis-encoded protein p37 can promote invasion of cancer cells in a dose-dependent manner, an effect that was blocked by monoclonal antibodies specific for p37. In this study, we further elucidated changes in growth, morphology and gene expression in prostate cancer cell lines when treated with exogenous p37 protein. Incubation with recombinant p37 caused significant nuclear enlargement, denoting active, anaplastic cells and increased the migratory potential of both PC-3 and DU145 cells. Microarray analysis of p37-treated and untreated cells identified eight gene expression clusters that could be broadly classified into three basic patterns. These were an increase in both cell lines, a decrease in either cell line or a cell line-specific differential trend. The most represented functional gene categories included cell cycle, signal transduction and metabolic factors. Taken together, these observations suggest that p37 potentiates the aggressiveness of prostate cancer and thus molecular events triggered by p37 maybe target for therapy.

Original languageEnglish (US)
Pages (from-to)204-213
Number of pages10
JournalCytogenetic and Genome Research
Volume118
Issue number2-4
DOIs
StatePublished - Nov 1 2007
Externally publishedYes

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Prostatic Neoplasms
Gene Expression
Cell Line
Growth
Mycoplasma hyorhinis
Proteins
Microarray Analysis
Multigene Family
Signal Transduction
Cell Cycle
Monoclonal Antibodies
Genes
Neoplasms
Therapeutics

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Exogenous mycoplasmal p37 protein alters gene expression, growth and morphology of prostate cancer cells. / Goodison, Steven; Nakamura, K.; Iczkowski, K. A.; Anai, S.; Boehlein, S. K.; Rosser, C. J.

In: Cytogenetic and Genome Research, Vol. 118, No. 2-4, 01.11.2007, p. 204-213.

Research output: Contribution to journalArticle

Goodison, Steven ; Nakamura, K. ; Iczkowski, K. A. ; Anai, S. ; Boehlein, S. K. ; Rosser, C. J. / Exogenous mycoplasmal p37 protein alters gene expression, growth and morphology of prostate cancer cells. In: Cytogenetic and Genome Research. 2007 ; Vol. 118, No. 2-4. pp. 204-213.
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