Exercise and metformin counteract altered mitochondrial function in the insulinresistant brain

Gregory N. Ruegsegger, Patrick M. Vanderboom, Surendra Dasari, Katherine A. Klaus, Parijat Kabiraj, Christina B. McCarthy, Claudia F. Lucchinetti, K. Sreekumaran Nair

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Insulin resistance associates with increased risk for cognitive decline and dementia; however, the underpinning mechanisms for this increased risk remain to be fully defined. As insulin resistance impairs mitochondrial oxidative metabolism and increases ROS in skeletal muscle, we considered whether similar events occur in the brain, which - like muscle - is rich in insulin receptors and mitochondria. We show that high-fat diet-induced (HFD-induced) brain insulin resistance in mice decreased mitochondrial ATP production rate and oxidative enzyme activities in brain regions rich in insulin receptors. HFD increased ROS emission and reduced antioxidant enzyme activities, with the concurrent accumulation of oxidatively damaged mitochondrial proteins and increased mitochondrial fission. Improvement of insulin sensitivity by both aerobic exercise and metformin ameliorated HFD-induced abnormalities. Moreover, insulin-induced enhancement of ATP production in primary cortical neurons and astrocytes was counteracted by the insulin receptor antagonist S961, demonstrating a direct effect of insulin resistance on brain mitochondria. Further, intranasal S961 administration prevented exercise-induced improvements in ATP production and ROS emission during HFD, supporting that exercise enhances brain mitochondrial function by improving insulin action. These results support that insulin sensitizing by exercise and metformin restores brain mitochondrial function in insulin-resistant states.

Original languageEnglish (US)
Article numbere130681
JournalJCI Insight
Volume4
Issue number18
DOIs
StatePublished - Sep 19 2019

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Metformin
Insulin Resistance
Insulin Receptor
Brain
Insulin
Adenosine Triphosphate
High Fat Diet
Insulin Antagonists
Mitochondria
Mitochondrial Dynamics
Intranasal Administration
Mitochondrial Proteins
Enzymes
Astrocytes
Dementia
Skeletal Muscle
Antioxidants
Exercise
Neurons
Muscles

ASJC Scopus subject areas

  • Medicine(all)

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Exercise and metformin counteract altered mitochondrial function in the insulinresistant brain. / Ruegsegger, Gregory N.; Vanderboom, Patrick M.; Dasari, Surendra; Klaus, Katherine A.; Kabiraj, Parijat; McCarthy, Christina B.; Lucchinetti, Claudia F.; Nair, K. Sreekumaran.

In: JCI Insight, Vol. 4, No. 18, e130681, 19.09.2019.

Research output: Contribution to journalArticle

Ruegsegger, Gregory N. ; Vanderboom, Patrick M. ; Dasari, Surendra ; Klaus, Katherine A. ; Kabiraj, Parijat ; McCarthy, Christina B. ; Lucchinetti, Claudia F. ; Nair, K. Sreekumaran. / Exercise and metformin counteract altered mitochondrial function in the insulinresistant brain. In: JCI Insight. 2019 ; Vol. 4, No. 18.
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