TY - JOUR
T1 - Exclusion of RNA strands from a purine motif triple helix
AU - Semerad, Craig L.
AU - James maher, L.
N1 - Funding Information:
We acknowledge the excellent technical assistance of R.Cerny, D.Eicher, C.Mountjoy, W.Olivas, C.McDonald, G.Soukupand J.Strauss. This work was supported in part by grant 5 P30 CA36727-08 from the National Cancer Institute and grant GM 47814 from the National Institutes of Health. The Midwest Center for Mass Spectrometry is partially supported by the National Science Foundation, Biology Division (grant no. DIR9017262). C.L.S was supported by the Summer Research Fellowship Program of the Eppley Institute. L.J.M. is a recipient of a Junior Faculty Research Award from the American Cancer Society and a Young Investigator's Award from Abbott Laboratories.
PY - 1994
Y1 - 1994
N2 - Research concerning oligonucleotide-directed triple helix formation has mainly focused on the binding of DNA oligonucleotides to duplex DNA. The participation of RNA strands In triple helices is also of interest. For the pyrimldine motif (pyrlmldlne- purine (pyrimldlne triplets), systematic substitution of RNA for DNA in one, two, or all three triplex strands has previously been reported. For the purine motif (purine-purine-pyrimldlne triplets), studies have shown only that RNA cannot bind to duplex DNA. To extend this result, we created a DNA triple helix in the purine motif and systematically replaced one, two, or all three strands with RNA. In dramatic contrast to the general accommodation of RNA strands in the pyrimidine triple helix motif, a stable triplex forms In the purine motif only when all three of the substltuent strands are DNA. The lack of triplex formation among any of the other seven possible strand combinations involving RNA suggests that: (i) duplex structures containing RNA cannot be targeted by DNA oligonucleotides In the purine motif; (ii) RNA strands cannot be employed to recognize duplex DNA in the purine motif; and (ill) RNA tertiary structures are likely to contain only isolated base triplets in the purine motif.
AB - Research concerning oligonucleotide-directed triple helix formation has mainly focused on the binding of DNA oligonucleotides to duplex DNA. The participation of RNA strands In triple helices is also of interest. For the pyrimldine motif (pyrlmldlne- purine (pyrimldlne triplets), systematic substitution of RNA for DNA in one, two, or all three triplex strands has previously been reported. For the purine motif (purine-purine-pyrimldlne triplets), studies have shown only that RNA cannot bind to duplex DNA. To extend this result, we created a DNA triple helix in the purine motif and systematically replaced one, two, or all three strands with RNA. In dramatic contrast to the general accommodation of RNA strands in the pyrimidine triple helix motif, a stable triplex forms In the purine motif only when all three of the substltuent strands are DNA. The lack of triplex formation among any of the other seven possible strand combinations involving RNA suggests that: (i) duplex structures containing RNA cannot be targeted by DNA oligonucleotides In the purine motif; (ii) RNA strands cannot be employed to recognize duplex DNA in the purine motif; and (ill) RNA tertiary structures are likely to contain only isolated base triplets in the purine motif.
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U2 - 10.1093/nar/22.24.5321
DO - 10.1093/nar/22.24.5321
M3 - Article
C2 - 7529405
AN - SCOPUS:0028631012
SN - 0305-1048
VL - 22
SP - 5321
EP - 5325
JO - Nucleic acids research
JF - Nucleic acids research
IS - 24
ER -