Excitatory input from the distal colon to the inferior mesenteric ganglion in the guinea‐pig

P. J. Crowcroft, Mollie E. Holman, J. H. Szurszewski

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Abstract

1. Intracellular recordings were obtained from ganglion cells in the guinea‐pig inferior mesenteric ganglion (IMG) with a segment of the distal colon attached to the lumbar colonic nerves. 2. Continuous electrical activity consisting of excitatory synaptic potentials and action potentials was recorded from ganglion cells in all regions of the IMG. 3. The ‘spontaneous’ synaptic potentials were indistinguishable from those elicited by submaximal stimulation of any of the nerve trunks connected to the IMG. 4. The excitatory activity was irreversibly abolished when the lumbar colonic nerves were cut and reversibly abolished when tetrodotoxin (5 × 10−7 g/ml.) was added to the colon side of a two‐compartment organ bath. 5. Addition of dihydro‐β‐erythroidine (5 × 10−6 g/ml.) to the ganglion side of the bath abolished the synaptic activity of colonic origin and the synaptic responses to stimulation of any of the nerve trunks connected to the IMG. 6. Addition of dihydro‐β‐erythroidine (1 × 10−5 g/ml.) to the colon side of the bath markedly depressed the synaptic input of colonic origin but had no effect on synaptic responses produced by preganglionic nerve stimulation. 7. Distension of the colonic segment and the application of 5‐HT (1 × 10−5 g/ml.) to the mucosal surface of the colon increased the frequency of synaptic input. 8. The synaptic input from the colon was transiently blocked following repetitive stimulation of any of the nerve trunks connected to the IMG. The discharge of miniature synaptic potentials was unaffected. 9. Addition of noradrenaline (1 × 10−7 to 1 × 10−6 g/ml.) to the colon side of the bath reduced, and in some cases completely abolished, the synaptic input to the IMG. Phentolamine (1 × 10−6 g/ml.), when added to the colon side of the bath, blocked the effect of noradrenaline and the transient inhibition following repetitive nerve stimulation. 10. Addition of noradrenaline (1 × 10−4 g/ml.) to the ganglion side of the bath reduced but never abolished the amplitude of the synaptic potentials of colonic origin. 11. It was concluded that in the guinea‐pig, the IMG is involved in a peripheral reflex whose afferent limit of this reflex consists of the axons of cholinergic neurones within the wall of the colon. Many of these neurones are driven either directly or indirectly by cholinergic synapses. The efferent noradrenergic neurones of the IMG function as a group of inhibitory neurones which depress the activity of the excitatory neurones of the colon which are driving them.

Original languageEnglish (US)
Pages (from-to)443-461
Number of pages19
JournalThe Journal of Physiology
Volume219
Issue number2
DOIs
StatePublished - Dec 1 1971

ASJC Scopus subject areas

  • Physiology

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