Exchange of viral promoter/enhancer elements with heterologous regulatory sequences generates targeted hybrid long terminal repeat vectors for gene therapy of melanoma

R. M. Diaz, T. Eisen, I. R. Hart, R. G. Vile

Research output: Contribution to journalArticle

57 Scopus citations


To generate transcriptionally targeted vectors, tissue-specific elements of the human tyrosinase promoter were exchanged with corresponding viral elements in the Moloney murine leukemia virus long terminal repeat (LTR). From these experiments, a vesicular stomatitis virus type G pseudotyped, hybrid LTR vector that contained three tyrosinase enhancer elements and gave high-level, tightly tissue-specific expression at high titers (3 x 107 CFU/ml) was constructed.

Original languageEnglish (US)
Pages (from-to)789-795
Number of pages7
JournalJournal of virology
Issue number1
StatePublished - Jan 1 1998


ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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