Evolution of Shear Stress, Protein Expression, and Vessel Area in an Animal Model of Arterial Dilatation in Hemodialysis Grafts

Sanjay Misra, Alex A. Fu, Khamal D. Misra, James Glockner, Debabrata Mukhopadyay

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Purpose: To evaluate the wall shear stress, protein expression of matrix metalloproteinase (MMP)-2 and MMP-9 and tissue inhibitor of matrix metalloproteinase (TIMP)-1 and TIMP-2, and vessel area over time in a porcine model for polytetrafluoroethylene (PTFE) hemodialysis grafts. Materials and Methods: In 21 pigs, subtotal renal infarction was created, and 28 days later, a PTFE graft was placed to connect the carotid artery to the ipsilateral jugular vein. Phase-contrast magnetic resonance imaging was used to measure blood flow and vessel area at 1, 3, 7, and 14 days after graft placement. Wall shear stress was estimated from the law of Poiseuille. Animals were killed at day 3 (n = 7), day 7 (n = 7), and day 14 (n = 7) and expression of MMP-2, MMP-9, TIMP-1, and TIMP-2 were determined at the grafted and control arteries. Results: The mean wall shear stress of the grafted artery was higher than in the control artery at all time points (P < .05). It peaked by day 3 and decreased by days 7-14 as the vessel area nearly doubled. By days 7-14, there was a significant increase in active MMP-2 followed by a significant increase in pro-MMP-9 and active MMP-9 by day 14 (P < .05, grafted artery vs control). TIMP-1 expression peaked by day 7 and then decreased, whereas TIMP-2 expression was decreased at days 7-14. Conclusions: The wall shear stress of the grafted artery peaks by day 3, with increased MMP-2 activity by days 7-14, followed by increase pro-MMP-9 and active MMP-9 by day 14. In addition, the vessel area nearly doubled.

Original languageEnglish (US)
Pages (from-to)108-115
Number of pages8
JournalJournal of Vascular and Interventional Radiology
Volume21
Issue number1
DOIs
StatePublished - Jan 2010

Fingerprint

Matrix Metalloproteinase 2
Heat-Shock Proteins
Renal Dialysis
Dilatation
Animal Models
Transplants
Matrix Metalloproteinase 9
Arteries
Tissue Inhibitor of Metalloproteinase-2
Matrix Metalloproteinase 1
Tissue Inhibitor of Metalloproteinase-1
Matrix Metalloproteinase Inhibitors
Polytetrafluoroethylene
Swine
Jugular Veins
Carotid Arteries
Infarction
Blood Vessels
Magnetic Resonance Imaging
Kidney

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Cardiology and Cardiovascular Medicine

Cite this

Evolution of Shear Stress, Protein Expression, and Vessel Area in an Animal Model of Arterial Dilatation in Hemodialysis Grafts. / Misra, Sanjay; Fu, Alex A.; Misra, Khamal D.; Glockner, James; Mukhopadyay, Debabrata.

In: Journal of Vascular and Interventional Radiology, Vol. 21, No. 1, 01.2010, p. 108-115.

Research output: Contribution to journalArticle

@article{abce277cc0534b3ebf3b1a2fe7c7add9,
title = "Evolution of Shear Stress, Protein Expression, and Vessel Area in an Animal Model of Arterial Dilatation in Hemodialysis Grafts",
abstract = "Purpose: To evaluate the wall shear stress, protein expression of matrix metalloproteinase (MMP)-2 and MMP-9 and tissue inhibitor of matrix metalloproteinase (TIMP)-1 and TIMP-2, and vessel area over time in a porcine model for polytetrafluoroethylene (PTFE) hemodialysis grafts. Materials and Methods: In 21 pigs, subtotal renal infarction was created, and 28 days later, a PTFE graft was placed to connect the carotid artery to the ipsilateral jugular vein. Phase-contrast magnetic resonance imaging was used to measure blood flow and vessel area at 1, 3, 7, and 14 days after graft placement. Wall shear stress was estimated from the law of Poiseuille. Animals were killed at day 3 (n = 7), day 7 (n = 7), and day 14 (n = 7) and expression of MMP-2, MMP-9, TIMP-1, and TIMP-2 were determined at the grafted and control arteries. Results: The mean wall shear stress of the grafted artery was higher than in the control artery at all time points (P < .05). It peaked by day 3 and decreased by days 7-14 as the vessel area nearly doubled. By days 7-14, there was a significant increase in active MMP-2 followed by a significant increase in pro-MMP-9 and active MMP-9 by day 14 (P < .05, grafted artery vs control). TIMP-1 expression peaked by day 7 and then decreased, whereas TIMP-2 expression was decreased at days 7-14. Conclusions: The wall shear stress of the grafted artery peaks by day 3, with increased MMP-2 activity by days 7-14, followed by increase pro-MMP-9 and active MMP-9 by day 14. In addition, the vessel area nearly doubled.",
author = "Sanjay Misra and Fu, {Alex A.} and Misra, {Khamal D.} and James Glockner and Debabrata Mukhopadyay",
year = "2010",
month = "1",
doi = "10.1016/j.jvir.2009.09.024",
language = "English (US)",
volume = "21",
pages = "108--115",
journal = "Journal of Vascular and Interventional Radiology",
issn = "1051-0443",
publisher = "Elsevier Inc.",
number = "1",

}

TY - JOUR

T1 - Evolution of Shear Stress, Protein Expression, and Vessel Area in an Animal Model of Arterial Dilatation in Hemodialysis Grafts

AU - Misra, Sanjay

AU - Fu, Alex A.

AU - Misra, Khamal D.

AU - Glockner, James

AU - Mukhopadyay, Debabrata

PY - 2010/1

Y1 - 2010/1

N2 - Purpose: To evaluate the wall shear stress, protein expression of matrix metalloproteinase (MMP)-2 and MMP-9 and tissue inhibitor of matrix metalloproteinase (TIMP)-1 and TIMP-2, and vessel area over time in a porcine model for polytetrafluoroethylene (PTFE) hemodialysis grafts. Materials and Methods: In 21 pigs, subtotal renal infarction was created, and 28 days later, a PTFE graft was placed to connect the carotid artery to the ipsilateral jugular vein. Phase-contrast magnetic resonance imaging was used to measure blood flow and vessel area at 1, 3, 7, and 14 days after graft placement. Wall shear stress was estimated from the law of Poiseuille. Animals were killed at day 3 (n = 7), day 7 (n = 7), and day 14 (n = 7) and expression of MMP-2, MMP-9, TIMP-1, and TIMP-2 were determined at the grafted and control arteries. Results: The mean wall shear stress of the grafted artery was higher than in the control artery at all time points (P < .05). It peaked by day 3 and decreased by days 7-14 as the vessel area nearly doubled. By days 7-14, there was a significant increase in active MMP-2 followed by a significant increase in pro-MMP-9 and active MMP-9 by day 14 (P < .05, grafted artery vs control). TIMP-1 expression peaked by day 7 and then decreased, whereas TIMP-2 expression was decreased at days 7-14. Conclusions: The wall shear stress of the grafted artery peaks by day 3, with increased MMP-2 activity by days 7-14, followed by increase pro-MMP-9 and active MMP-9 by day 14. In addition, the vessel area nearly doubled.

AB - Purpose: To evaluate the wall shear stress, protein expression of matrix metalloproteinase (MMP)-2 and MMP-9 and tissue inhibitor of matrix metalloproteinase (TIMP)-1 and TIMP-2, and vessel area over time in a porcine model for polytetrafluoroethylene (PTFE) hemodialysis grafts. Materials and Methods: In 21 pigs, subtotal renal infarction was created, and 28 days later, a PTFE graft was placed to connect the carotid artery to the ipsilateral jugular vein. Phase-contrast magnetic resonance imaging was used to measure blood flow and vessel area at 1, 3, 7, and 14 days after graft placement. Wall shear stress was estimated from the law of Poiseuille. Animals were killed at day 3 (n = 7), day 7 (n = 7), and day 14 (n = 7) and expression of MMP-2, MMP-9, TIMP-1, and TIMP-2 were determined at the grafted and control arteries. Results: The mean wall shear stress of the grafted artery was higher than in the control artery at all time points (P < .05). It peaked by day 3 and decreased by days 7-14 as the vessel area nearly doubled. By days 7-14, there was a significant increase in active MMP-2 followed by a significant increase in pro-MMP-9 and active MMP-9 by day 14 (P < .05, grafted artery vs control). TIMP-1 expression peaked by day 7 and then decreased, whereas TIMP-2 expression was decreased at days 7-14. Conclusions: The wall shear stress of the grafted artery peaks by day 3, with increased MMP-2 activity by days 7-14, followed by increase pro-MMP-9 and active MMP-9 by day 14. In addition, the vessel area nearly doubled.

UR - http://www.scopus.com/inward/record.url?scp=72249083903&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=72249083903&partnerID=8YFLogxK

U2 - 10.1016/j.jvir.2009.09.024

DO - 10.1016/j.jvir.2009.09.024

M3 - Article

C2 - 20123196

AN - SCOPUS:72249083903

VL - 21

SP - 108

EP - 115

JO - Journal of Vascular and Interventional Radiology

JF - Journal of Vascular and Interventional Radiology

SN - 1051-0443

IS - 1

ER -