This chapter describes several deconvolution approaches that we have developed. These were specifically designed to interpret pulsatile hormone time series observations. Such endocrine data are unique in that there is a limited number of observations with a large amount of experimental uncertainty. These unique features preclude the use of most earlier deconvolution techniques that require large numbers of data points and negligible experimental uncertainties. The PULSE2 program is available for 80386-80387, or faster microprocessors, for IBM-compatible computers on written request from the authors.