Evidence that Less-than-Full-Length pol Gene Products Are Functional in Hepadnavirus DNA Synthesis

Tsung Teh Wu, Lynn D. Condreay, Laura Coates, Carol Aldrich, William Mason

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Abstract

Duck hepatitis B virus mutants containing frameshift or stop codon mutations in a portion of the viral pol gene separating the terminal protein and reverse transcriptase domains had a leaky phenotype and, depending on the location and type of mutation, synthesized up to 10% as much viral DNA as did the wild type. This region of the pol gene had previously been reported to be refractory to missense mutations; in fact, the leakiness of most of our mutants appeared attributable to translational suppression, which would also be expected to introduce amino acid changes. However, at least one mutant (pH1093+2), which was ca. 10% as active as the wild type, appeared to use a novel pathway to express the viral pol gene. Our analyses indicated that pH1093+2 synthesized the viral reverse transcriptase as a fusion protein with the amino-terminal portion of the pre-S envelope protein. Thus, in this case, the products of the terminal-protein and reverse transcriptase domains of the pol gene would function as separate protein species, though perhaps noncovalently joined in a dimeric structure during assembly of DNA replication complexes. Evidence was also obtained that was consistent with the idea that the wild-type pol gene may, at least in certain instances, be expressed as functional, subgenic polypeptides.

Original languageEnglish (US)
Pages (from-to)2155-2163
Number of pages9
JournalJournal of Virology
Volume65
Issue number5
StatePublished - May 1991
Externally publishedYes

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ASJC Scopus subject areas

  • Immunology

Cite this

Wu, T. T., Condreay, L. D., Coates, L., Aldrich, C., & Mason, W. (1991). Evidence that Less-than-Full-Length pol Gene Products Are Functional in Hepadnavirus DNA Synthesis. Journal of Virology, 65(5), 2155-2163.