TY - JOUR
T1 - Evidence that high-density lipoprotein cholesterol is an independent predictor of acute platelet-dependent thrombus formation
AU - Naqvi, Tasneem Z.
AU - Shah, Prediman K.
AU - Ivey, Pamela A.
AU - Molloy, Mia D.
AU - Thomas, Arun M.
AU - Panicker, Sandeep
AU - Ahmed, Alaa
AU - Cercek, Bojan
AU - Kaul, Sanjay
N1 - Copyright:
Copyright 2007 Elsevier B.V., All rights reserved.
PY - 1999/11/1
Y1 - 1999/11/1
N2 - Plasma total and low-density lipoprotein (LDL) cholesterol are established risk factors for atherosclerotic vascular disease and may also contribute to a prothrombotic risk via enhanced platelet reactivity. This study examines whether high-density lipoprotein (HDL) cholesterol, which is inversely correlated with coronary artery disease, is associated with a reduced thrombogenic potential. Platelet thrombus formation was evaluated by exposing porcine aortic media placed in Badimon perfusion chambers to flowing nonanticoagulated venous blood for 5 minutes at a shear rate of 1,000 s-1. Forty-five subjects, 23 normal (LDL 104 ± 31, HDL 50 ± 15 mg/dl) and 22 hypercholesterolemic (LDL 181 ± 45, HDL 41 ± 10 mg/dl) patients without coronary artery disease were studied. Platelet aggregation and CD62 antigen expression, and assay for circulating prothrombotic factors were also performed. In univariate analysis platelet thrombus formation correlated with weight (r = 0.33, p = 0.03), diastolic blood pressure (r = 0.39, p = 0.01), HDL cholesterol (r = -0.45, p = 0.003), total/HDL cholesterol (r = 0.43, p = 0.004) and LDL/HDL (r = 0.38, p = 0.01) ratios, and platelet CD62 expression (r = 0.41, p = 0.02). In multiple regression analysis only HDL cholesterol showed significant correlation with platelet thrombus formation (p = 0.03). Platelet aggregation and circulating prothrombotic factors did not correlate with platelet thrombus formation. A comparison between normal and hypercholesterolemic subjects revealed enhanced thrombus area (0.026 ± 0.20 vs 0.045 ± 0.039 mm2/mm; p = 0.04), resting CD62 expression (6 ± 7% vs 15 ± 10% positive platelets, p = 0.02), and platelet aggregation (16.7 ± 5.2 vs 21.7 ± 6.7 ohms, p = 0.04) in hypercholesterolemic subjects. Our results demonstrate that HDL cholesterol is a significant independent predictor of ex vivo platelet thrombus formation.
AB - Plasma total and low-density lipoprotein (LDL) cholesterol are established risk factors for atherosclerotic vascular disease and may also contribute to a prothrombotic risk via enhanced platelet reactivity. This study examines whether high-density lipoprotein (HDL) cholesterol, which is inversely correlated with coronary artery disease, is associated with a reduced thrombogenic potential. Platelet thrombus formation was evaluated by exposing porcine aortic media placed in Badimon perfusion chambers to flowing nonanticoagulated venous blood for 5 minutes at a shear rate of 1,000 s-1. Forty-five subjects, 23 normal (LDL 104 ± 31, HDL 50 ± 15 mg/dl) and 22 hypercholesterolemic (LDL 181 ± 45, HDL 41 ± 10 mg/dl) patients without coronary artery disease were studied. Platelet aggregation and CD62 antigen expression, and assay for circulating prothrombotic factors were also performed. In univariate analysis platelet thrombus formation correlated with weight (r = 0.33, p = 0.03), diastolic blood pressure (r = 0.39, p = 0.01), HDL cholesterol (r = -0.45, p = 0.003), total/HDL cholesterol (r = 0.43, p = 0.004) and LDL/HDL (r = 0.38, p = 0.01) ratios, and platelet CD62 expression (r = 0.41, p = 0.02). In multiple regression analysis only HDL cholesterol showed significant correlation with platelet thrombus formation (p = 0.03). Platelet aggregation and circulating prothrombotic factors did not correlate with platelet thrombus formation. A comparison between normal and hypercholesterolemic subjects revealed enhanced thrombus area (0.026 ± 0.20 vs 0.045 ± 0.039 mm2/mm; p = 0.04), resting CD62 expression (6 ± 7% vs 15 ± 10% positive platelets, p = 0.02), and platelet aggregation (16.7 ± 5.2 vs 21.7 ± 6.7 ohms, p = 0.04) in hypercholesterolemic subjects. Our results demonstrate that HDL cholesterol is a significant independent predictor of ex vivo platelet thrombus formation.
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U2 - 10.1016/S0002-9149(99)00489-0
DO - 10.1016/S0002-9149(99)00489-0
M3 - Article
C2 - 10569655
AN - SCOPUS:0032699713
SN - 0002-9149
VL - 84
SP - 1011
EP - 1017
JO - American Journal of Cardiology
JF - American Journal of Cardiology
IS - 9
ER -