Evidence that high-density lipoprotein cholesterol is an independent predictor of acute platelet-dependent thrombus formation

Tasneem Zehra Naqvi, Prediman K. Shah, Pamela A. Ivey, Mia D. Molloy, Arun M. Thomas, Sandeep Panicker, Alaa Ahmed, Bojan Cercek, Sanjay Kaul

Research output: Contribution to journalArticle

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Abstract

Plasma total and low-density lipoprotein (LDL) cholesterol are established risk factors for atherosclerotic vascular disease and may also contribute to a prothrombotic risk via enhanced platelet reactivity. This study examines whether high-density lipoprotein (HDL) cholesterol, which is inversely correlated with coronary artery disease, is associated with a reduced thrombogenic potential. Platelet thrombus formation was evaluated by exposing porcine aortic media placed in Badimon perfusion chambers to flowing nonanticoagulated venous blood for 5 minutes at a shear rate of 1,000 s-1. Forty-five subjects, 23 normal (LDL 104 ± 31, HDL 50 ± 15 mg/dl) and 22 hypercholesterolemic (LDL 181 ± 45, HDL 41 ± 10 mg/dl) patients without coronary artery disease were studied. Platelet aggregation and CD62 antigen expression, and assay for circulating prothrombotic factors were also performed. In univariate analysis platelet thrombus formation correlated with weight (r = 0.33, p = 0.03), diastolic blood pressure (r = 0.39, p = 0.01), HDL cholesterol (r = -0.45, p = 0.003), total/HDL cholesterol (r = 0.43, p = 0.004) and LDL/HDL (r = 0.38, p = 0.01) ratios, and platelet CD62 expression (r = 0.41, p = 0.02). In multiple regression analysis only HDL cholesterol showed significant correlation with platelet thrombus formation (p = 0.03). Platelet aggregation and circulating prothrombotic factors did not correlate with platelet thrombus formation. A comparison between normal and hypercholesterolemic subjects revealed enhanced thrombus area (0.026 ± 0.20 vs 0.045 ± 0.039 mm2/mm; p = 0.04), resting CD62 expression (6 ± 7% vs 15 ± 10% positive platelets, p = 0.02), and platelet aggregation (16.7 ± 5.2 vs 21.7 ± 6.7 ohms, p = 0.04) in hypercholesterolemic subjects. Our results demonstrate that HDL cholesterol is a significant independent predictor of ex vivo platelet thrombus formation.

Original languageEnglish (US)
Pages (from-to)1011-1017
Number of pages7
JournalAmerican Journal of Cardiology
Volume84
Issue number9
DOIs
StatePublished - Nov 1 1999
Externally publishedYes

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HDL Cholesterol
Thrombosis
Blood Platelets
HDL Lipoproteins
Platelet Aggregation
LDL Lipoproteins
Coronary Artery Disease
Blood Pressure
Vascular Diseases
LDL Cholesterol
Swine
Perfusion
Regression Analysis
Antigens
Weights and Measures

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Evidence that high-density lipoprotein cholesterol is an independent predictor of acute platelet-dependent thrombus formation. / Naqvi, Tasneem Zehra; Shah, Prediman K.; Ivey, Pamela A.; Molloy, Mia D.; Thomas, Arun M.; Panicker, Sandeep; Ahmed, Alaa; Cercek, Bojan; Kaul, Sanjay.

In: American Journal of Cardiology, Vol. 84, No. 9, 01.11.1999, p. 1011-1017.

Research output: Contribution to journalArticle

Naqvi, Tasneem Zehra ; Shah, Prediman K. ; Ivey, Pamela A. ; Molloy, Mia D. ; Thomas, Arun M. ; Panicker, Sandeep ; Ahmed, Alaa ; Cercek, Bojan ; Kaul, Sanjay. / Evidence that high-density lipoprotein cholesterol is an independent predictor of acute platelet-dependent thrombus formation. In: American Journal of Cardiology. 1999 ; Vol. 84, No. 9. pp. 1011-1017.
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abstract = "Plasma total and low-density lipoprotein (LDL) cholesterol are established risk factors for atherosclerotic vascular disease and may also contribute to a prothrombotic risk via enhanced platelet reactivity. This study examines whether high-density lipoprotein (HDL) cholesterol, which is inversely correlated with coronary artery disease, is associated with a reduced thrombogenic potential. Platelet thrombus formation was evaluated by exposing porcine aortic media placed in Badimon perfusion chambers to flowing nonanticoagulated venous blood for 5 minutes at a shear rate of 1,000 s-1. Forty-five subjects, 23 normal (LDL 104 ± 31, HDL 50 ± 15 mg/dl) and 22 hypercholesterolemic (LDL 181 ± 45, HDL 41 ± 10 mg/dl) patients without coronary artery disease were studied. Platelet aggregation and CD62 antigen expression, and assay for circulating prothrombotic factors were also performed. In univariate analysis platelet thrombus formation correlated with weight (r = 0.33, p = 0.03), diastolic blood pressure (r = 0.39, p = 0.01), HDL cholesterol (r = -0.45, p = 0.003), total/HDL cholesterol (r = 0.43, p = 0.004) and LDL/HDL (r = 0.38, p = 0.01) ratios, and platelet CD62 expression (r = 0.41, p = 0.02). In multiple regression analysis only HDL cholesterol showed significant correlation with platelet thrombus formation (p = 0.03). Platelet aggregation and circulating prothrombotic factors did not correlate with platelet thrombus formation. A comparison between normal and hypercholesterolemic subjects revealed enhanced thrombus area (0.026 ± 0.20 vs 0.045 ± 0.039 mm2/mm; p = 0.04), resting CD62 expression (6 ± 7{\%} vs 15 ± 10{\%} positive platelets, p = 0.02), and platelet aggregation (16.7 ± 5.2 vs 21.7 ± 6.7 ohms, p = 0.04) in hypercholesterolemic subjects. Our results demonstrate that HDL cholesterol is a significant independent predictor of ex vivo platelet thrombus formation.",
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AU - Shah, Prediman K.

AU - Ivey, Pamela A.

AU - Molloy, Mia D.

AU - Thomas, Arun M.

AU - Panicker, Sandeep

AU - Ahmed, Alaa

AU - Cercek, Bojan

AU - Kaul, Sanjay

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N2 - Plasma total and low-density lipoprotein (LDL) cholesterol are established risk factors for atherosclerotic vascular disease and may also contribute to a prothrombotic risk via enhanced platelet reactivity. This study examines whether high-density lipoprotein (HDL) cholesterol, which is inversely correlated with coronary artery disease, is associated with a reduced thrombogenic potential. Platelet thrombus formation was evaluated by exposing porcine aortic media placed in Badimon perfusion chambers to flowing nonanticoagulated venous blood for 5 minutes at a shear rate of 1,000 s-1. Forty-five subjects, 23 normal (LDL 104 ± 31, HDL 50 ± 15 mg/dl) and 22 hypercholesterolemic (LDL 181 ± 45, HDL 41 ± 10 mg/dl) patients without coronary artery disease were studied. Platelet aggregation and CD62 antigen expression, and assay for circulating prothrombotic factors were also performed. In univariate analysis platelet thrombus formation correlated with weight (r = 0.33, p = 0.03), diastolic blood pressure (r = 0.39, p = 0.01), HDL cholesterol (r = -0.45, p = 0.003), total/HDL cholesterol (r = 0.43, p = 0.004) and LDL/HDL (r = 0.38, p = 0.01) ratios, and platelet CD62 expression (r = 0.41, p = 0.02). In multiple regression analysis only HDL cholesterol showed significant correlation with platelet thrombus formation (p = 0.03). Platelet aggregation and circulating prothrombotic factors did not correlate with platelet thrombus formation. A comparison between normal and hypercholesterolemic subjects revealed enhanced thrombus area (0.026 ± 0.20 vs 0.045 ± 0.039 mm2/mm; p = 0.04), resting CD62 expression (6 ± 7% vs 15 ± 10% positive platelets, p = 0.02), and platelet aggregation (16.7 ± 5.2 vs 21.7 ± 6.7 ohms, p = 0.04) in hypercholesterolemic subjects. Our results demonstrate that HDL cholesterol is a significant independent predictor of ex vivo platelet thrombus formation.

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