Evidence of steroid receptors in human osteoblast-like cells

Douglas Colvard, Thomas Spelsberg, Erik Eriksen, Philip Keeting, B. Lawrence Riggs

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

A nuclear binding assay was used to demonstrate steroid receptors in normal human osteoblast-like cells. Nuclear binding of [3H] estradiol was found in 27 of 30 (90% cell strains and nuclear binding of [3H] androgen was identified in 21 of 25 (84% separate osteoblast cell strains. The nuclear binding was saturable and steroid-specific. Estrogen and androgen receptor gene expression was confirmed by RNA blot analysis. These data suggest that circulating sex steroids may act directly on normal human osteoblasts through receptor-mediated mechanisms.

Original languageEnglish (US)
Pages (from-to)33-40
Number of pages8
JournalConnective Tissue Research
Volume20
Issue number1-4
DOIs
StatePublished - 1989

Fingerprint

Steroid Receptors
Osteoblasts
Steroids
Androgen Receptors
Gene expression
Estrogen Receptors
Androgens
Estradiol
Assays
RNA
Gene Expression

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology
  • Orthopedics and Sports Medicine
  • Rheumatology

Cite this

Colvard, D., Spelsberg, T., Eriksen, E., Keeting, P., & Riggs, B. L. (1989). Evidence of steroid receptors in human osteoblast-like cells. Connective Tissue Research, 20(1-4), 33-40. https://doi.org/10.3109/03008208909023872

Evidence of steroid receptors in human osteoblast-like cells. / Colvard, Douglas; Spelsberg, Thomas; Eriksen, Erik; Keeting, Philip; Riggs, B. Lawrence.

In: Connective Tissue Research, Vol. 20, No. 1-4, 1989, p. 33-40.

Research output: Contribution to journalArticle

Colvard, D, Spelsberg, T, Eriksen, E, Keeting, P & Riggs, BL 1989, 'Evidence of steroid receptors in human osteoblast-like cells', Connective Tissue Research, vol. 20, no. 1-4, pp. 33-40. https://doi.org/10.3109/03008208909023872
Colvard, Douglas ; Spelsberg, Thomas ; Eriksen, Erik ; Keeting, Philip ; Riggs, B. Lawrence. / Evidence of steroid receptors in human osteoblast-like cells. In: Connective Tissue Research. 1989 ; Vol. 20, No. 1-4. pp. 33-40.
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