Evidence of steroid receptors in human osteoblast-like cells

Douglas Colvard, Thomas Spelsberg, Erik Eriksen, Philip Keeting, B. Lawrence Riggs

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

A nuclear binding assay was used to demonstrate steroid receptors in normal human osteoblast-like cells. Nuclear binding of [3H] estradiol was found in 27 of 30 (90% cell strains and nuclear binding of [3H] androgen was identified in 21 of 25 (84% separate osteoblast cell strains. The nuclear binding was saturable and steroid-specific. Estrogen and androgen receptor gene expression was confirmed by RNA blot analysis. These data suggest that circulating sex steroids may act directly on normal human osteoblasts through receptor-mediated mechanisms.

Original languageEnglish (US)
Pages (from-to)33-40
Number of pages8
JournalConnective Tissue Research
Volume20
Issue number1-4
DOIs
StatePublished - Jan 1 1989

ASJC Scopus subject areas

  • Rheumatology
  • Biochemistry
  • Orthopedics and Sports Medicine
  • Molecular Biology
  • Cell Biology

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    Colvard, D., Spelsberg, T., Eriksen, E., Keeting, P., & Riggs, B. L. (1989). Evidence of steroid receptors in human osteoblast-like cells. Connective Tissue Research, 20(1-4), 33-40. https://doi.org/10.3109/03008208909023872