Evidence of steroid receptors in human osteoblast-like cells

Douglas Colvard; Thomas Spelsberg; Erik Eriksen; Philip Keeting; B. Lawrence Riggs

doi:10.3109/03008208909023872

Evidence of steroid receptors in human osteoblast-like cells

Douglas Colvard, Thomas Spelsberg, Erik Eriksen, Philip Keeting, B. Lawrence Riggs

Biochemistry and Molecular Biology

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

A nuclear binding assay was used to demonstrate steroid receptors in normal human osteoblast-like cells. Nuclear binding of [³H] estradiol was found in 27 of 30 (90% cell strains and nuclear binding of [³H] androgen was identified in 21 of 25 (84% separate osteoblast cell strains. The nuclear binding was saturable and steroid-specific. Estrogen and androgen receptor gene expression was confirmed by RNA blot analysis. These data suggest that circulating sex steroids may act directly on normal human osteoblasts through receptor-mediated mechanisms.

Original language	English (US)
Pages (from-to)	33-40
Number of pages	8
Journal	Connective Tissue Research
Volume	20
Issue number	1-4
DOIs	https://doi.org/10.3109/03008208909023872
State	Published - 1989

ASJC Scopus subject areas

Rheumatology
Biochemistry
Orthopedics and Sports Medicine
Molecular Biology
Cell Biology

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abstract = "A nuclear binding assay was used to demonstrate steroid receptors in normal human osteoblast-like cells. Nuclear binding of [3H] estradiol was found in 27 of 30 (90% cell strains and nuclear binding of [3H] androgen was identified in 21 of 25 (84% separate osteoblast cell strains. The nuclear binding was saturable and steroid-specific. Estrogen and androgen receptor gene expression was confirmed by RNA blot analysis. These data suggest that circulating sex steroids may act directly on normal human osteoblasts through receptor-mediated mechanisms.",

author = "Douglas Colvard and Thomas Spelsberg and Erik Eriksen and Philip Keeting and Riggs, {B. Lawrence}",

year = "1989",

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AU - Spelsberg, Thomas

AU - Eriksen, Erik

AU - Keeting, Philip

AU - Riggs, B. Lawrence

PY - 1989

Y1 - 1989

N2 - A nuclear binding assay was used to demonstrate steroid receptors in normal human osteoblast-like cells. Nuclear binding of [3H] estradiol was found in 27 of 30 (90% cell strains and nuclear binding of [3H] androgen was identified in 21 of 25 (84% separate osteoblast cell strains. The nuclear binding was saturable and steroid-specific. Estrogen and androgen receptor gene expression was confirmed by RNA blot analysis. These data suggest that circulating sex steroids may act directly on normal human osteoblasts through receptor-mediated mechanisms.

AB - A nuclear binding assay was used to demonstrate steroid receptors in normal human osteoblast-like cells. Nuclear binding of [3H] estradiol was found in 27 of 30 (90% cell strains and nuclear binding of [3H] androgen was identified in 21 of 25 (84% separate osteoblast cell strains. The nuclear binding was saturable and steroid-specific. Estrogen and androgen receptor gene expression was confirmed by RNA blot analysis. These data suggest that circulating sex steroids may act directly on normal human osteoblasts through receptor-mediated mechanisms.

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Evidence of steroid receptors in human osteoblast-like cells

Abstract

ASJC Scopus subject areas

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