Evidence of nanobacterial-like structures in calcified human arteries and cardiac valves

Virginia M Miller, George Rodgers, Jon A. Charlesworth, Brenda Kirkland, Sandra R. Severson, Todd E. Rasmussen, Marineh Yagubyan, Jeri C. Rodgers, Franklin R. Cockerill, Robert L. Folk, Ewa Rzewuska-Lech, Vivek Kumar, Gerard Farell-Baril, John C Lieske

Research output: Contribution to journalArticle

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Abstract

Mechanisms mediating vascular calcification remain incompletely understood. Nanometer scale objects hypothesized to be a type of bacteria (nanobacteria) are associated with calcified geological specimens, human kidney stones, and psammona bodies in ovarian cancer. Experiments were designed to evaluate human vascular tissue for the presence of similar nanometer-scale objects. Calcified human aneurysms (n = 8), carotid plaques (n = 2), femoral arterial plaques (n = 2), and cardiac valves (n = 2) and noncalcified aneurysms from patients with bicuspid aortic valve disease (n = 2) were collected as surgical waste from the Heart Hospital of Austin, Austin, Texas, and Mayo Clinic, Rochester, Minnesota. Whole mounts or adjacent sections from each specimen were examined by electron microscopy, stained for calcium phosphate, or stained with a commercially available antibody (8D10). Filtered (0.2 μm) homogenates of aneurysms were cultured and costained with 8D10 antibody followed by PicoGreen to detect DNA or incubated with [3H]uridine. Staining for calcium phosphate was heterogeneously distributed within all calcified tissues. Immunological staining with 8D10 was also heterogeneously distributed in areas with and without calcium phosphate. Analysis of areas with positive immunostaining identified spheres ranging in size from 30 to 100 nm with a spectral pattern of calcium and phosphorus (high-energy dispersive spectroscopy). Nanosized particles cultured from calcified but not from noncalcified aneurysms were recognized by a DNA-specific dye and incorporated radiolabeled uridine, and, after decalcification, they appeared via electron microscopy to contain cell walls. Therefore, nanometer-scale particles similar to those described as nanobacteria isolated from geological specimens and human kidney stones can be visualized in and cultured from calcified human cardiovascular tissue.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume287
Issue number3 56-3
DOIs
StatePublished - Sep 2004

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Heart Valves
Arteries
Aneurysm
Calcifying Nanoparticles
Kidney Calculi
Uridine
Electron Microscopy
Staining and Labeling
Vascular Calcification
Aortic Diseases
Antibodies
DNA
Thigh
Ovarian Neoplasms
Cell Wall
Phosphorus
Blood Vessels
Spectrum Analysis
Coloring Agents
Calcium

Keywords

  • Aneurysm
  • Chlamydia
  • Infection
  • Inflammation
  • Nanobacteria

ASJC Scopus subject areas

  • Physiology

Cite this

Evidence of nanobacterial-like structures in calcified human arteries and cardiac valves. / Miller, Virginia M; Rodgers, George; Charlesworth, Jon A.; Kirkland, Brenda; Severson, Sandra R.; Rasmussen, Todd E.; Yagubyan, Marineh; Rodgers, Jeri C.; Cockerill, Franklin R.; Folk, Robert L.; Rzewuska-Lech, Ewa; Kumar, Vivek; Farell-Baril, Gerard; Lieske, John C.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 287, No. 3 56-3, 09.2004.

Research output: Contribution to journalArticle

Miller, VM, Rodgers, G, Charlesworth, JA, Kirkland, B, Severson, SR, Rasmussen, TE, Yagubyan, M, Rodgers, JC, Cockerill, FR, Folk, RL, Rzewuska-Lech, E, Kumar, V, Farell-Baril, G & Lieske, JC 2004, 'Evidence of nanobacterial-like structures in calcified human arteries and cardiac valves', American Journal of Physiology - Heart and Circulatory Physiology, vol. 287, no. 3 56-3. https://doi.org/10.1152/ajpheart.00075.2004
Miller, Virginia M ; Rodgers, George ; Charlesworth, Jon A. ; Kirkland, Brenda ; Severson, Sandra R. ; Rasmussen, Todd E. ; Yagubyan, Marineh ; Rodgers, Jeri C. ; Cockerill, Franklin R. ; Folk, Robert L. ; Rzewuska-Lech, Ewa ; Kumar, Vivek ; Farell-Baril, Gerard ; Lieske, John C. / Evidence of nanobacterial-like structures in calcified human arteries and cardiac valves. In: American Journal of Physiology - Heart and Circulatory Physiology. 2004 ; Vol. 287, No. 3 56-3.
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