The authors demonstrated in an anti Ia serum the presence of specific antibodies reacting within T cells, as well as with B cells, using a highly sensitive dye exclusion test. This antiserum reacts with both spleen and lymph node in a characteristic biphasic titration curve killing up to 70% of these cells. It also reacts with cortisone resistant thymocytes. The A.TH α A.TL serum can be absorbed with spleen, lymph node, cortisone resistant thymus, or normal thymus cells. Further in vivo absorptions in BALB/c nude cannot remove all of the cytotoxic activity for normal BALB lymph node lymphocytes, while completely removing the activity for nude cells. A Thy 1 positive cell line derived from a C57Br leukemia is reactive with this anti Ia serum.
|Original language||English (US)|
|Number of pages||12|
|Journal||Journal of Experimental Medicine|
|State||Published - 1974|
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