Evidence for the expression of Ia (H 2 associated) antigens on thymus derived lymphocytes

J. A. Frelinger, J. E. Niederhuber, C. S. David, D. C. Shreffler

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Abstract

The authors demonstrated in an anti Ia serum the presence of specific antibodies reacting within T cells, as well as with B cells, using a highly sensitive dye exclusion test. This antiserum reacts with both spleen and lymph node in a characteristic biphasic titration curve killing up to 70% of these cells. It also reacts with cortisone resistant thymocytes. The A.TH α A.TL serum can be absorbed with spleen, lymph node, cortisone resistant thymus, or normal thymus cells. Further in vivo absorptions in BALB/c nude cannot remove all of the cytotoxic activity for normal BALB lymph node lymphocytes, while completely removing the activity for nude cells. A Thy 1 positive cell line derived from a C57Br leukemia is reactive with this anti Ia serum.

Original languageEnglish (US)
Pages (from-to)1273-1284
Number of pages12
JournalJournal of Experimental Medicine
Volume140
Issue number5
StatePublished - 1974
Externally publishedYes

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ASJC Scopus subject areas

  • Immunology

Cite this

Frelinger, J. A., Niederhuber, J. E., David, C. S., & Shreffler, D. C. (1974). Evidence for the expression of Ia (H 2 associated) antigens on thymus derived lymphocytes. Journal of Experimental Medicine, 140(5), 1273-1284.