Ia antigenic specificities determined by the I-A subregion of the mouse major histocompatibility complex have been examined in strain B10.D2 (H 2(d)), C57BL/10 (H 2(b)), and in a (C57BL/6 x DBA/2) hybrid (BDF1; H 2(b/d)). Detergent solubilized, 3H leucine labeled antigen preparations were mixed with appropriate alloantisera and precipitation was induced either by addition of goat anti mouse γ globulin or by addition of protein A bearing staphylococci. Sequential precipitation analysis showed that in strain B10.D2, Ia specificities 8 and 11 were co precipitable, and that in strain C57BL/10, Ia specificities 8 and 9 were co precipitable. In contrast, precipitation of specificities 9 and 11 from a BDF1 antigen preparation showed that these two Ia specificities were on separate molecules. The genetic implications of these data are discussed.
|Original language||English (US)|
|Number of pages||5|
|Journal||Journal of Immunology|
|State||Published - Dec 1 1976|
ASJC Scopus subject areas
- Immunology and Allergy