Evidence for more than one Ia antigenic specificity on molecules determined by the I-A subregion of the mouse major histocompatibility complex

S. E. Cullen, C. S. David, J. L. Cone, D. H. Sachs

Research output: Contribution to journalArticle

29 Scopus citations

Abstract

Ia antigenic specificities determined by the I-A subregion of the mouse major histocompatibility complex have been examined in strain B10.D2 (H 2(d)), C57BL/10 (H 2(b)), and in a (C57BL/6 x DBA/2) hybrid (BDF1; H 2(b/d)). Detergent solubilized, 3H leucine labeled antigen preparations were mixed with appropriate alloantisera and precipitation was induced either by addition of goat anti mouse γ globulin or by addition of protein A bearing staphylococci. Sequential precipitation analysis showed that in strain B10.D2, Ia specificities 8 and 11 were co precipitable, and that in strain C57BL/10, Ia specificities 8 and 9 were co precipitable. In contrast, precipitation of specificities 9 and 11 from a BDF1 antigen preparation showed that these two Ia specificities were on separate molecules. The genetic implications of these data are discussed.

Original languageEnglish (US)
Pages (from-to)549-553
Number of pages5
JournalJournal of Immunology
Volume116
Issue number2
StatePublished - Dec 1 1976

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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