TY - JOUR
T1 - Evidence for a functionally active inhibitory guanine nucleotide-binding regulatory protein in the swine ovary
AU - Veldhuis, Johannes D.
AU - Hewlett, Erik L.
N1 - Funding Information:
We thank Chris McNett for skillful preparation of the manuscript, Smithfield Packing Corp. for the provision of swine ovaries, and Paula P. Asimi for the artwork. This work was supported in part by RCDA *l K04 HD00634, ROlHD16806, ROlHD16393 (JDV), A118000, the Rockefeller Foundation, NIH Biomedical Research Support Grant 5S07RR05431-21, and the University of Virginia Pratt Fund (ELH).
PY - 1985/9/30
Y1 - 1985/9/30
N2 - Incubation of particulate fractions of swine granulosa cells or luteal minces with purified pertussis toxin (islet-activitating protein) and [32P]-NAD catalyzed the (32P)-ADP ribosylation of a 41,000 dalton membrane protein. ADP-ribosylation was markedly reduced by prior incubation of intact cells with toxin. The functional relevance of this presumptive inhibitory guanine nucleotide-binding protein in pig granulosa cells was indicated by the ability of prior treatment with pertussis toxin to increase cyclic AMP generation and progesterone production significantly in response to follicle stimulating hormone. Prior cellular intoxication also enhanced cyclic AMP production stimulated by luteinizing hormone and choleratoxin, but not basally or after forskolin. these results demonstrate the presence of an inhibitory guanine nucleotide-binding protein in both the follicular (granulosa cell) and luteal compartments of the mammalian ovary, and indicate its functional relevance in cyclic AMP generation and progesterone secretion.
AB - Incubation of particulate fractions of swine granulosa cells or luteal minces with purified pertussis toxin (islet-activitating protein) and [32P]-NAD catalyzed the (32P)-ADP ribosylation of a 41,000 dalton membrane protein. ADP-ribosylation was markedly reduced by prior incubation of intact cells with toxin. The functional relevance of this presumptive inhibitory guanine nucleotide-binding protein in pig granulosa cells was indicated by the ability of prior treatment with pertussis toxin to increase cyclic AMP generation and progesterone production significantly in response to follicle stimulating hormone. Prior cellular intoxication also enhanced cyclic AMP production stimulated by luteinizing hormone and choleratoxin, but not basally or after forskolin. these results demonstrate the presence of an inhibitory guanine nucleotide-binding protein in both the follicular (granulosa cell) and luteal compartments of the mammalian ovary, and indicate its functional relevance in cyclic AMP generation and progesterone secretion.
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U2 - 10.1016/0006-291X(85)90213-X
DO - 10.1016/0006-291X(85)90213-X
M3 - Article
C2 - 2996539
AN - SCOPUS:0022346790
SN - 0006-291X
VL - 131
SP - 1168
EP - 1174
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -