Evidence for a frizzled-mediated wnt pathway required for zebrafish dorsal mesoderm formation

Aidas Nasevicius, Tana Hyatt, Hyon Kim, Julie Guttman, Erin Walsh, Saulius Sumanas, Yanshu Wang, Stephen C. Ekker

Research output: Contribution to journalArticle

43 Scopus citations

Abstract

We have used zebrafish as a model system for the study of vertebrate dorsoventral patterning. We isolated a maternally expressed and dorsal organizer localized member of the frizzled family of wnt receptors. Wild-type and dominant, loss-of-function molecules in misexpression studies demonstrate frizzled function is necessary and sufficient for dorsal mesoderm specification. frizzled activity is antagonized by the action of GSK-3, and we show GSK-3 is also required for zebrafish dorsal mesoderm formation. frizzled cooperatively interacts with the maternally encoded zebrafish wnt8 protein in dorsal mesodermal fate determination. This frizzled -mediated wnt pathway for dorsal mesoderm specification provides the first evidence for the requirement of a wnt-like signal in vertebrate axis determination.

Original languageEnglish (US)
Pages (from-to)4283-4292
Number of pages10
JournalDevelopment
Volume125
Issue number21
StatePublished - Dec 2 1998

Keywords

  • Dorsal organizer
  • Frizzled
  • Mesoderm Dorsoventral patterning
  • Zebrafish
  • wnt signalling

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology

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    Nasevicius, A., Hyatt, T., Kim, H., Guttman, J., Walsh, E., Sumanas, S., Wang, Y., & Ekker, S. C. (1998). Evidence for a frizzled-mediated wnt pathway required for zebrafish dorsal mesoderm formation. Development, 125(21), 4283-4292.