Evidence for a frizzled-mediated wnt pathway required for zebrafish dorsal mesoderm formation

Aidas Nasevicius, Tana Hyatt, Hyon Kim, Julie Guttman, Erin Walsh, Saulius Sumanas, Yanshu Wang, Stephen C Ekker

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

We have used zebrafish as a model system for the study of vertebrate dorsoventral patterning. We isolated a maternally expressed and dorsal organizer localized member of the frizzled family of wnt receptors. Wild-type and dominant, loss-of-function molecules in misexpression studies demonstrate frizzled function is necessary and sufficient for dorsal mesoderm specification. frizzled activity is antagonized by the action of GSK-3, and we show GSK-3 is also required for zebrafish dorsal mesoderm formation. frizzled cooperatively interacts with the maternally encoded zebrafish wnt8 protein in dorsal mesodermal fate determination. This frizzled -mediated wnt pathway for dorsal mesoderm specification provides the first evidence for the requirement of a wnt-like signal in vertebrate axis determination.

Original languageEnglish (US)
Pages (from-to)4283-4292
Number of pages10
JournalDevelopment
Volume125
Issue number21
StatePublished - 1998
Externally publishedYes

Fingerprint

Wnt Signaling Pathway
Mesoderm
Zebrafish
Glycogen Synthase Kinase 3
Vertebrates
Zebrafish Proteins
Wnt Receptors

Keywords

  • Dorsal organizer
  • Frizzled
  • Mesoderm Dorsoventral patterning
  • wnt signalling
  • Zebrafish

ASJC Scopus subject areas

  • Cell Biology
  • Anatomy

Cite this

Nasevicius, A., Hyatt, T., Kim, H., Guttman, J., Walsh, E., Sumanas, S., ... Ekker, S. C. (1998). Evidence for a frizzled-mediated wnt pathway required for zebrafish dorsal mesoderm formation. Development, 125(21), 4283-4292.

Evidence for a frizzled-mediated wnt pathway required for zebrafish dorsal mesoderm formation. / Nasevicius, Aidas; Hyatt, Tana; Kim, Hyon; Guttman, Julie; Walsh, Erin; Sumanas, Saulius; Wang, Yanshu; Ekker, Stephen C.

In: Development, Vol. 125, No. 21, 1998, p. 4283-4292.

Research output: Contribution to journalArticle

Nasevicius, A, Hyatt, T, Kim, H, Guttman, J, Walsh, E, Sumanas, S, Wang, Y & Ekker, SC 1998, 'Evidence for a frizzled-mediated wnt pathway required for zebrafish dorsal mesoderm formation', Development, vol. 125, no. 21, pp. 4283-4292.
Nasevicius A, Hyatt T, Kim H, Guttman J, Walsh E, Sumanas S et al. Evidence for a frizzled-mediated wnt pathway required for zebrafish dorsal mesoderm formation. Development. 1998;125(21):4283-4292.
Nasevicius, Aidas ; Hyatt, Tana ; Kim, Hyon ; Guttman, Julie ; Walsh, Erin ; Sumanas, Saulius ; Wang, Yanshu ; Ekker, Stephen C. / Evidence for a frizzled-mediated wnt pathway required for zebrafish dorsal mesoderm formation. In: Development. 1998 ; Vol. 125, No. 21. pp. 4283-4292.
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