TY - JOUR
T1 - Evaluation of tumor angiogenesis measured with microvessel density (MVD) as a prognostic indicator in nasopharyngeal carcinoma
T2 - Results of RTOG 9505
AU - Foote, Robert L.
AU - Weidner, Noel
AU - Harris, Jonathan
AU - Hammond, Elizabeth
AU - Lewis, Jean E.
AU - Vuong, Te
AU - Ang, K. Kian
AU - Fu, Karen K.
N1 - Funding Information:
Supported by grants U10 CA21661, U10 CA37422, and U10 CA32115 from the National Cancer Institute.
PY - 2005/3/1
Y1 - 2005/3/1
N2 - Purpose: The objective of this study was to evaluate tumor angiogenesis as measured by microvessel density (MVD) as an independent prognostic factor in patients with nasopharyngeal carcinoma (NPC) treated with radiotherapy alone. Methods and Materials: Eligible patients included those with NPC treated with radiotherapy. Paraffin blocks of the primary tumor had a hematoxylin and eosin-stained section prepared at the block face. One representative section for tumor was stained for factor VIII-related antigen using a standard immunoperoxidase staining technique. MVD was determined by light microscopy in areas of invasive tumor containing the highest numbers of capillaries and microvessels per area. Individual microvessel counts were made on a 200× field within the area of most intense tumor neovascularization. Results were expressed as the highest number of microvessels identified within any single 200× field. Using a breakpoint of MVD <60 vs. <60, the distributions between the two MVD groups were compared by the method of Gray. Overall survival rates were estimated by the Kaplan-Meier method and compared by the log-rank test. A multivariate Cox proportional hazard model was employed to examine the relationship between MVD and disease outcomes while adjusting for other concomitant variables. Results: One hundred sixty-six were eligible, of whom 123 had values determined for MVD. The MVD values ranged from 9 to 243 with a median of 70. In the multivariate analysis of overall survival, distant metastases, and local-regional failure, MVD did not significantly improve the model containing T stage, N stage, age, radiation dose, and World Health Organization class. Conclusions: We found no significant differences in overall survival, time to distant metastasis, or time to local-regional failure using a breakpoint of MVD <60 vs. MVD ≥60. The study was perhaps limited by the small size of the NPC samples.
AB - Purpose: The objective of this study was to evaluate tumor angiogenesis as measured by microvessel density (MVD) as an independent prognostic factor in patients with nasopharyngeal carcinoma (NPC) treated with radiotherapy alone. Methods and Materials: Eligible patients included those with NPC treated with radiotherapy. Paraffin blocks of the primary tumor had a hematoxylin and eosin-stained section prepared at the block face. One representative section for tumor was stained for factor VIII-related antigen using a standard immunoperoxidase staining technique. MVD was determined by light microscopy in areas of invasive tumor containing the highest numbers of capillaries and microvessels per area. Individual microvessel counts were made on a 200× field within the area of most intense tumor neovascularization. Results were expressed as the highest number of microvessels identified within any single 200× field. Using a breakpoint of MVD <60 vs. <60, the distributions between the two MVD groups were compared by the method of Gray. Overall survival rates were estimated by the Kaplan-Meier method and compared by the log-rank test. A multivariate Cox proportional hazard model was employed to examine the relationship between MVD and disease outcomes while adjusting for other concomitant variables. Results: One hundred sixty-six were eligible, of whom 123 had values determined for MVD. The MVD values ranged from 9 to 243 with a median of 70. In the multivariate analysis of overall survival, distant metastases, and local-regional failure, MVD did not significantly improve the model containing T stage, N stage, age, radiation dose, and World Health Organization class. Conclusions: We found no significant differences in overall survival, time to distant metastasis, or time to local-regional failure using a breakpoint of MVD <60 vs. MVD ≥60. The study was perhaps limited by the small size of the NPC samples.
KW - Angiogenesis
KW - Microvessel density
KW - Nasopharyngeal carcinoma
KW - Radiotherapy
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U2 - 10.1016/j.ijrobp.2004.07.694
DO - 10.1016/j.ijrobp.2004.07.694
M3 - Article
C2 - 15708253
AN - SCOPUS:13844254877
SN - 0360-3016
VL - 61
SP - 745
EP - 753
JO - International Journal of Radiation Oncology Biology Physics
JF - International Journal of Radiation Oncology Biology Physics
IS - 3
ER -