Evaluation of the Bruker Biotyper and Vitek MS matrix-assisted laser desorption ionization-time of flight mass spectrometry systems for identification of nonfermenting gram-negative bacilli isolated from cultures from cystic fibrosis patients

Daniel C. Marko, Ryan T. Saffert, Scott A. Cunningham, Jay Hyman, John Walsh, Sophie Arbefeville, Wanita Howard, Jon Pruessner, Nedal Safwat, Franklin R. Cockerill, Aaron D. Bossler, Robin Patel, Sandra S. Richter

Research output: Contribution to journalArticle

80 Citations (Scopus)

Abstract

The Bruker Biotyper and Vitek MS matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry (MS) instruments were evaluated for the identification of nonfermenting Gram-negative bacilli (NFGNB) by a blinded comparison to conventional biochemical or molecular methods. Two hundred NFGNB that were recovered from cultures from cystic fibrosis patients in the University of Iowa Health Care (UIHC) Microbiology Laboratory between 1 January 2006 and 31 October 2010 were sent to Mayo Clinic for analysis with the Bruker Biotyper (software version 3.0) and to bioMérieux for testing with Vitek MS (SARAMIS database version 3.62). If two attempts at direct colony testing failed to provide an acceptable MALDI-TOF identification, an extraction procedure was performed. The MS identifications from both of these systems were provided to UIHC for comparison to the biochemical or molecular identification that had been reported in the patient record. Isolates with discordant results were analyzed by 16S rRNA gene sequencing at UIHC. After discrepancy testing, the Bruker Biotyper result agreed with the biochemical or molecular method, with 72.5% of isolates to the species level, 5.5% to the complex level, and 19% to the genus level (3% not identified). The level of agreement for Vitek MS was 80% species, 3.5% complex, 6% genus, and 3.5% family (7% not identified). Both MS systems provided rapid (≤3 min per isolate) and reliable identifications. The agreement of combined species/complex/genus-level identification with the reference method was higher for the Bruker Biotyper (97% versus 89.5%, P=0.004) but required an extraction step more often. Species-level agreement with the reference method was similar for both MS systems (72.5% and 80%, P=0.099).

Original languageEnglish (US)
Pages (from-to)2034-2039
Number of pages6
JournalJournal of Clinical Microbiology
Volume50
Issue number6
DOIs
StatePublished - Jun 2012

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Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Cystic Fibrosis
Bacillus
Mass Spectrometry
Delivery of Health Care
Microbiology
rRNA Genes
Lasers
Software
Databases

ASJC Scopus subject areas

  • Microbiology (medical)

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Evaluation of the Bruker Biotyper and Vitek MS matrix-assisted laser desorption ionization-time of flight mass spectrometry systems for identification of nonfermenting gram-negative bacilli isolated from cultures from cystic fibrosis patients. / Marko, Daniel C.; Saffert, Ryan T.; Cunningham, Scott A.; Hyman, Jay; Walsh, John; Arbefeville, Sophie; Howard, Wanita; Pruessner, Jon; Safwat, Nedal; Cockerill, Franklin R.; Bossler, Aaron D.; Patel, Robin; Richter, Sandra S.

In: Journal of Clinical Microbiology, Vol. 50, No. 6, 06.2012, p. 2034-2039.

Research output: Contribution to journalArticle

Marko, Daniel C. ; Saffert, Ryan T. ; Cunningham, Scott A. ; Hyman, Jay ; Walsh, John ; Arbefeville, Sophie ; Howard, Wanita ; Pruessner, Jon ; Safwat, Nedal ; Cockerill, Franklin R. ; Bossler, Aaron D. ; Patel, Robin ; Richter, Sandra S. / Evaluation of the Bruker Biotyper and Vitek MS matrix-assisted laser desorption ionization-time of flight mass spectrometry systems for identification of nonfermenting gram-negative bacilli isolated from cultures from cystic fibrosis patients. In: Journal of Clinical Microbiology. 2012 ; Vol. 50, No. 6. pp. 2034-2039.
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abstract = "The Bruker Biotyper and Vitek MS matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry (MS) instruments were evaluated for the identification of nonfermenting Gram-negative bacilli (NFGNB) by a blinded comparison to conventional biochemical or molecular methods. Two hundred NFGNB that were recovered from cultures from cystic fibrosis patients in the University of Iowa Health Care (UIHC) Microbiology Laboratory between 1 January 2006 and 31 October 2010 were sent to Mayo Clinic for analysis with the Bruker Biotyper (software version 3.0) and to bioM{\'e}rieux for testing with Vitek MS (SARAMIS database version 3.62). If two attempts at direct colony testing failed to provide an acceptable MALDI-TOF identification, an extraction procedure was performed. The MS identifications from both of these systems were provided to UIHC for comparison to the biochemical or molecular identification that had been reported in the patient record. Isolates with discordant results were analyzed by 16S rRNA gene sequencing at UIHC. After discrepancy testing, the Bruker Biotyper result agreed with the biochemical or molecular method, with 72.5{\%} of isolates to the species level, 5.5{\%} to the complex level, and 19{\%} to the genus level (3{\%} not identified). The level of agreement for Vitek MS was 80{\%} species, 3.5{\%} complex, 6{\%} genus, and 3.5{\%} family (7{\%} not identified). Both MS systems provided rapid (≤3 min per isolate) and reliable identifications. The agreement of combined species/complex/genus-level identification with the reference method was higher for the Bruker Biotyper (97{\%} versus 89.5{\%}, P=0.004) but required an extraction step more often. Species-level agreement with the reference method was similar for both MS systems (72.5{\%} and 80{\%}, P=0.099).",
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AU - Saffert, Ryan T.

AU - Cunningham, Scott A.

AU - Hyman, Jay

AU - Walsh, John

AU - Arbefeville, Sophie

AU - Howard, Wanita

AU - Pruessner, Jon

AU - Safwat, Nedal

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AU - Bossler, Aaron D.

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