Evaluation of osteoconductive scaffolds in the canine femoral multi-defect model

Viviane Luangphakdy, Esteban Walker, Kentaro Shinohara, Hui Pan, Theresa Hefferan, Thomas W. Bauer, Linda Stockdale, Sunil Saini, Mahrokh Dadsetan, M. Brett Runge, Amit Vasanji, Linda Griffith, Michael J Yaszemski, George F. Muschler

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Treatment of large segmental bone defects remains an unsolved clinical challenge, despite a wide array of existing bone graft materials. This project was designed to rapidly assess and compare promising biodegradable osteoconductive scaffolds for use in the systematic development of new bone regeneration methodologies that combine scaffolds, sources of osteogenic cells, and bioactive scaffold modifications. Promising biomaterials and scaffold fabrication methods were identified in laboratories at Rutgers, MIT, Integra Life Sciences, and Mayo Clinic. Scaffolds were fabricated from various materials, including poly(L-lactide-co-glycolide) (PLGA), poly(L-lactide-co- ε-caprolactone) (PLCL), tyrosine-derived polycarbonate (TyrPC), and poly(propylene fumarate) (PPF). Highly porous three-dimensional (3D) scaffolds were fabricated by 3D printing, laser stereolithography, or solvent casting followed by porogen leaching. The canine femoral multi-defect model was used to systematically compare scaffold performance and enable selection of the most promising substrate(s) on which to add cell sourcing options and bioactive surface modifications. Mineralized cancellous allograft (MCA) was used to provide a comparative reference to the current clinical standard for osteoconductive scaffolds. Percent bone volume within the defect was assessed 4 weeks after implantation using both MicroCT and limited histomorphometry. Bone formed at the periphery of all scaffolds with varying levels of radial ingrowth. MCA produced a rapid and advanced stage of bone formation and remodeling throughout the defect in 4 weeks, greatly exceeding the performance of all polymer scaffolds. Two scaffold constructs, TyrPCPL/TCP and PPF4 SLA/HAPLGA Dip, proved to be significantly better than alternative PLGA and PLCL scaffolds, justifying further development. MCA remains the current standard for osteoconductive scaffolds.

Original languageEnglish (US)
Pages (from-to)634-648
Number of pages15
JournalTissue Engineering - Part A
Volume19
Issue number5-6
DOIs
StatePublished - Mar 1 2013

Fingerprint

Thigh
Scaffolds
Canidae
Allografts
Bone and Bones
Defects
polycarbonate
Bone
Polyglactin 910
X-Ray Microtomography
Bone Regeneration
Biological Science Disciplines
Bone Remodeling
Biocompatible Materials
Osteogenesis
Tyrosine
Polymers
Lasers
Transplants
poly(lactide)

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Biomedical Engineering
  • Biomaterials
  • Medicine(all)

Cite this

Luangphakdy, V., Walker, E., Shinohara, K., Pan, H., Hefferan, T., Bauer, T. W., ... Muschler, G. F. (2013). Evaluation of osteoconductive scaffolds in the canine femoral multi-defect model. Tissue Engineering - Part A, 19(5-6), 634-648. https://doi.org/10.1089/ten.tea.2012.0289

Evaluation of osteoconductive scaffolds in the canine femoral multi-defect model. / Luangphakdy, Viviane; Walker, Esteban; Shinohara, Kentaro; Pan, Hui; Hefferan, Theresa; Bauer, Thomas W.; Stockdale, Linda; Saini, Sunil; Dadsetan, Mahrokh; Runge, M. Brett; Vasanji, Amit; Griffith, Linda; Yaszemski, Michael J; Muschler, George F.

In: Tissue Engineering - Part A, Vol. 19, No. 5-6, 01.03.2013, p. 634-648.

Research output: Contribution to journalArticle

Luangphakdy, V, Walker, E, Shinohara, K, Pan, H, Hefferan, T, Bauer, TW, Stockdale, L, Saini, S, Dadsetan, M, Runge, MB, Vasanji, A, Griffith, L, Yaszemski, MJ & Muschler, GF 2013, 'Evaluation of osteoconductive scaffolds in the canine femoral multi-defect model', Tissue Engineering - Part A, vol. 19, no. 5-6, pp. 634-648. https://doi.org/10.1089/ten.tea.2012.0289
Luangphakdy V, Walker E, Shinohara K, Pan H, Hefferan T, Bauer TW et al. Evaluation of osteoconductive scaffolds in the canine femoral multi-defect model. Tissue Engineering - Part A. 2013 Mar 1;19(5-6):634-648. https://doi.org/10.1089/ten.tea.2012.0289
Luangphakdy, Viviane ; Walker, Esteban ; Shinohara, Kentaro ; Pan, Hui ; Hefferan, Theresa ; Bauer, Thomas W. ; Stockdale, Linda ; Saini, Sunil ; Dadsetan, Mahrokh ; Runge, M. Brett ; Vasanji, Amit ; Griffith, Linda ; Yaszemski, Michael J ; Muschler, George F. / Evaluation of osteoconductive scaffolds in the canine femoral multi-defect model. In: Tissue Engineering - Part A. 2013 ; Vol. 19, No. 5-6. pp. 634-648.
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