Evaluation of mRNA by Q-RTPCR and protein expression by AQUA of the M2 subunit of ribonucleotide reductase (RRM2) in human tumors

Jill Kolesar, Wei Huang, Jens Eickhoff, Kristine Hahn, Dona Alberti, Steven Attia, William Schelman, Kyle Holen, Anne Traynor, Percy Ivy, George Wilding

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

Purpose: The purpose of this study was to evaluate baseline RRM2 protein and gene expression in tumors of patients receiving 3-AP. Methods: Tumor blocks from patients enrolled in phase I and II clinical studies using 3-AP, were evaluated for RRM2 gene and protein expression by quantitative real time polymerase chain reaction (Q-RTPCR) and automated quantitative analysis (AQUA). Results: Esophageal and gastric cancers overexpressed RRM2 protein when compared to prostate cancer (Z-score, 0.68 ± 0.94 SD, vs 0.41 ± 0.84 SD, respectively; p = 0.04). Esophageal and gastric cancers also overexpressed RRM2 mRNA when compared to prostate cancer (relative gene expression 2.56 ± 1.49 SD, vs 0.29 ± 0.20 SD, respectively; p = 0.02). Protein and gene expression were moderately associated (Spearman's rank correlation = 0.30; p = 0.12). Conclusion: RRM2 gene and protein expression varies by tumor type.

Original languageEnglish (US)
Pages (from-to)79-86
Number of pages8
JournalCancer chemotherapy and pharmacology
Volume64
Issue number1
DOIs
StatePublished - Jun 1 2009

Keywords

  • 3-Aminopyridine-2-carboxaldehyde thiosemicarbazone
  • Automated quantitative immunohistochemistry (AQUA)
  • Quantitative real time PCR
  • Ribonucleotide reductase
  • Triapine

ASJC Scopus subject areas

  • Oncology
  • Toxicology
  • Pharmacology
  • Cancer Research
  • Pharmacology (medical)

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    Kolesar, J., Huang, W., Eickhoff, J., Hahn, K., Alberti, D., Attia, S., Schelman, W., Holen, K., Traynor, A., Ivy, P., & Wilding, G. (2009). Evaluation of mRNA by Q-RTPCR and protein expression by AQUA of the M2 subunit of ribonucleotide reductase (RRM2) in human tumors. Cancer chemotherapy and pharmacology, 64(1), 79-86. https://doi.org/10.1007/s00280-008-0845-0