Evaluation of coronary adventitial vasa vasorum using 3D optical coherence tomography - Animal and human studies

Tatsuo Aoki, Martin G Rodriguez-Porcel, Yoshiki Matsuo, Andrew Cassar, Teak Geun Kwon, Federico Franchi, Rajiv Gulati, Sudhir S. Kushwaha, Ryan J. Lennon, Lilach O Lerman, Erik L. Ritman, Amir Lerman

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Objectives: This study sought to evaluate adventitial vasa vasorum (VV) invivo with novel imaging technique of optical coherence tomography (OCT). Methods: To verify OCT methods for quantification of VV, we first studied 2 swine carotid arteries in a model of focal angiogenesis by autologous blood injection, and compared microchannel volume (MCV) by OCT and VV by m-CT, and counts of those. In OCT images, adventitial MC was identified as signal-voiding areas which were located within 1mm from the lumen-intima border. After manually tracing microchannel areas and the boundaries of lumen-intima and media-adventitial in all slices, we reconstructed 3D images. Moreover, we performed with OCT imaging in 8 recipients referred for evaluation of cardiac allograft vasculopathy at 1 year after heart transplantation. MCV and plaque volume (PV) were assessed with 3D images in each 10-mm-segment. Results: In the animal study, among the 16 corresponding 1-mm-segments, there were significant correlations of count and volume between both the modalities (count r2=0.80, P<0.01; volume r2=0.50, P<0.01) and a good agreement with a systemic bias toward underestimation with m-CT. In the human study, there was a significant positive correlation between MCV and PV (segment number=24, r2=0.63, P<0.01). Conclusion: Our results suggest that evaluation of MCV with 3D OCT imaging might be a novel method to estimate the amount of adventitial VV invivo, and further has the potential to provide a pathophysiological insight into a role of the VV in allograft vasculopathy.

Original languageEnglish (US)
Pages (from-to)203-208
Number of pages6
JournalAtherosclerosis
Volume239
Issue number1
DOIs
StatePublished - Mar 1 2015

Fingerprint

Vasa Vasorum
Adventitia
Optical Coherence Tomography
Allografts
Heart Transplantation
Carotid Arteries
Swine
Injections

Keywords

  • Cardiac allograft vasculopathy
  • Microchannel
  • Microvessel
  • Optical coherence tomography

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Evaluation of coronary adventitial vasa vasorum using 3D optical coherence tomography - Animal and human studies. / Aoki, Tatsuo; Rodriguez-Porcel, Martin G; Matsuo, Yoshiki; Cassar, Andrew; Kwon, Teak Geun; Franchi, Federico; Gulati, Rajiv; Kushwaha, Sudhir S.; Lennon, Ryan J.; Lerman, Lilach O; Ritman, Erik L.; Lerman, Amir.

In: Atherosclerosis, Vol. 239, No. 1, 01.03.2015, p. 203-208.

Research output: Contribution to journalArticle

Aoki, Tatsuo ; Rodriguez-Porcel, Martin G ; Matsuo, Yoshiki ; Cassar, Andrew ; Kwon, Teak Geun ; Franchi, Federico ; Gulati, Rajiv ; Kushwaha, Sudhir S. ; Lennon, Ryan J. ; Lerman, Lilach O ; Ritman, Erik L. ; Lerman, Amir. / Evaluation of coronary adventitial vasa vasorum using 3D optical coherence tomography - Animal and human studies. In: Atherosclerosis. 2015 ; Vol. 239, No. 1. pp. 203-208.
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AU - Aoki, Tatsuo

AU - Rodriguez-Porcel, Martin G

AU - Matsuo, Yoshiki

AU - Cassar, Andrew

AU - Kwon, Teak Geun

AU - Franchi, Federico

AU - Gulati, Rajiv

AU - Kushwaha, Sudhir S.

AU - Lennon, Ryan J.

AU - Lerman, Lilach O

AU - Ritman, Erik L.

AU - Lerman, Amir

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N2 - Objectives: This study sought to evaluate adventitial vasa vasorum (VV) invivo with novel imaging technique of optical coherence tomography (OCT). Methods: To verify OCT methods for quantification of VV, we first studied 2 swine carotid arteries in a model of focal angiogenesis by autologous blood injection, and compared microchannel volume (MCV) by OCT and VV by m-CT, and counts of those. In OCT images, adventitial MC was identified as signal-voiding areas which were located within 1mm from the lumen-intima border. After manually tracing microchannel areas and the boundaries of lumen-intima and media-adventitial in all slices, we reconstructed 3D images. Moreover, we performed with OCT imaging in 8 recipients referred for evaluation of cardiac allograft vasculopathy at 1 year after heart transplantation. MCV and plaque volume (PV) were assessed with 3D images in each 10-mm-segment. Results: In the animal study, among the 16 corresponding 1-mm-segments, there were significant correlations of count and volume between both the modalities (count r2=0.80, P<0.01; volume r2=0.50, P<0.01) and a good agreement with a systemic bias toward underestimation with m-CT. In the human study, there was a significant positive correlation between MCV and PV (segment number=24, r2=0.63, P<0.01). Conclusion: Our results suggest that evaluation of MCV with 3D OCT imaging might be a novel method to estimate the amount of adventitial VV invivo, and further has the potential to provide a pathophysiological insight into a role of the VV in allograft vasculopathy.

AB - Objectives: This study sought to evaluate adventitial vasa vasorum (VV) invivo with novel imaging technique of optical coherence tomography (OCT). Methods: To verify OCT methods for quantification of VV, we first studied 2 swine carotid arteries in a model of focal angiogenesis by autologous blood injection, and compared microchannel volume (MCV) by OCT and VV by m-CT, and counts of those. In OCT images, adventitial MC was identified as signal-voiding areas which were located within 1mm from the lumen-intima border. After manually tracing microchannel areas and the boundaries of lumen-intima and media-adventitial in all slices, we reconstructed 3D images. Moreover, we performed with OCT imaging in 8 recipients referred for evaluation of cardiac allograft vasculopathy at 1 year after heart transplantation. MCV and plaque volume (PV) were assessed with 3D images in each 10-mm-segment. Results: In the animal study, among the 16 corresponding 1-mm-segments, there were significant correlations of count and volume between both the modalities (count r2=0.80, P<0.01; volume r2=0.50, P<0.01) and a good agreement with a systemic bias toward underestimation with m-CT. In the human study, there was a significant positive correlation between MCV and PV (segment number=24, r2=0.63, P<0.01). Conclusion: Our results suggest that evaluation of MCV with 3D OCT imaging might be a novel method to estimate the amount of adventitial VV invivo, and further has the potential to provide a pathophysiological insight into a role of the VV in allograft vasculopathy.

KW - Cardiac allograft vasculopathy

KW - Microchannel

KW - Microvessel

KW - Optical coherence tomography

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