Abstract
The rapid increase in the number of DNA-based clinical diagnostic procedures, particularly polymerase chain reaction (PCR)-based assays, has generated interest in analytical techniques that are less time-consuming and labor-intensive than traditional procedures such as polyacrylamide gel electrophoresis (PAGE). Capillary electrophoresis with laser-induced fluorescence (CE-LIF) detection, which allows for rapid and sensitive detection of DNA fragments in an automated format, is well-suited for DNA-based clinical assays. In this study, we demonstrate the potential of CE-LIF for the detection of PCR products from T-cell receptor gamma (TCRγ) gene rearrangements present in monoclonal populations of lymphocytes. The presence of monoclonal populations of T-cells is associated (but not always synonymous) with lymphocytic malignancies. Analysis of 31 patient samples, as well as sensitivity controls, demonstrated that CE-LIF detection of monoclonal lymphocytic populations is comparable to that of PAGE-SYBR Green I staining, and that CE-LIF detection can be accomplished in less than 20 min. These preliminary results illustrate the potential feasibility of a CE-based diagnostic assay for the detection of T-cell gene rearrangements.
Original language | English (US) |
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Pages (from-to) | 1491-1498 |
Number of pages | 8 |
Journal | ELECTROPHORESIS |
Volume | 17 |
Issue number | 9 |
DOIs | |
State | Published - Sep 1996 |
Keywords
- Capillary electrophoresis
- Gene rearrangements
- Laser-induced fluorescence
- Polymer solutions
- Polymerase chain reaction
- T-cell receptor (TCRγ)
ASJC Scopus subject areas
- Analytical Chemistry
- Biochemistry
- Clinical Biochemistry