Evaluation of adult equine bone marrow- and adipose-derived progenitor cell chondrogenesis in hydrogel cultures

John D. Kisiday, Paul W. Kopesky, Christopher H. Evans, Alan J. Grodzinsky, C. Wayne Mcllwraith, David D. Frisbie

Research output: Contribution to journalArticle

163 Scopus citations

Abstract

Bone marrow mesenchymal stem cells (BM-MSCs) and adipose-derived progenitor cells (ADPCs) are potential alternatives to autologous chondrocytes for cartilage resurfacing strategies. In this study, the chondrogenic potentials of these cell types were compared by quantifying neo-tissue synthesis and assaying gene expression and accumulation of extracellular matrix (ECM) components of cartilage. Adult equine progenitor cells encapsulated in agarose or self-assembling peptide hydrogels were cultured in the presence or absence of TGFβ1 for 3 weeks. In BM-MSCs-seeded hydrogels, TGFβl stimulated ECM synthesis and accumulation 3-41-fold relative to TGFβ1-free culture. In ADPC cultures, TGFβl stimulated a significant increase in ECM synthesis and accumulation in peptide (18-29-fold) but not agarose hydrogels. Chromato-graphic analysis of BM-MSC-seeded agarose and peptide hydrogels cultured in TGFβl medium showed extensive synthesis of aggrecan-like proteoglycan monomers. ADPCs seeded in peptide hydrogel also synthesized aggrecan-like proteoglycans, although to a lesser extent than seen in BM-MSC hydrogels, whereas aggrecan-like proteoglycan synthesis in ADPC-seeded agarose was minimal. RT-PCR analysis of TGFβl cultures showed detectable levels of type II collagen gene expression in BM-MSC but not ADPC cultures. Histological analysis of TGFβl-cultured peptide hydrogels showed the deposition of a continuous proteoglycan- and type II collagen rich ECM for BM-MSCs but not ADPCs. Therefore, this study showed both protein and gene expression evidence of superior chondrogenesis of BM-MSCs relative to ADPCs.

Original languageEnglish (US)
Pages (from-to)322-331
Number of pages10
JournalJournal of Orthopaedic Research
Volume26
Issue number3
DOIs
StatePublished - Mar 1 2008

Keywords

  • Chondrogenesis
  • Progenitor cells
  • Tissue engineering

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine

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