Evaluation of adult equine bone marrow- and adipose-derived progenitor cell chondrogenesis in hydrogel cultures

John D. Kisiday, Paul W. Kopesky, Christopher H Evans, Alan J. Grodzinsky, C. Wayne Mcllwraith, David D. Frisbie

Research output: Contribution to journalArticle

154 Citations (Scopus)

Abstract

Bone marrow mesenchymal stem cells (BM-MSCs) and adipose-derived progenitor cells (ADPCs) are potential alternatives to autologous chondrocytes for cartilage resurfacing strategies. In this study, the chondrogenic potentials of these cell types were compared by quantifying neo-tissue synthesis and assaying gene expression and accumulation of extracellular matrix (ECM) components of cartilage. Adult equine progenitor cells encapsulated in agarose or self-assembling peptide hydrogels were cultured in the presence or absence of TGFβ1 for 3 weeks. In BM-MSCs-seeded hydrogels, TGFβl stimulated ECM synthesis and accumulation 3-41-fold relative to TGFβ1-free culture. In ADPC cultures, TGFβl stimulated a significant increase in ECM synthesis and accumulation in peptide (18-29-fold) but not agarose hydrogels. Chromato-graphic analysis of BM-MSC-seeded agarose and peptide hydrogels cultured in TGFβl medium showed extensive synthesis of aggrecan-like proteoglycan monomers. ADPCs seeded in peptide hydrogel also synthesized aggrecan-like proteoglycans, although to a lesser extent than seen in BM-MSC hydrogels, whereas aggrecan-like proteoglycan synthesis in ADPC-seeded agarose was minimal. RT-PCR analysis of TGFβl cultures showed detectable levels of type II collagen gene expression in BM-MSC but not ADPC cultures. Histological analysis of TGFβl-cultured peptide hydrogels showed the deposition of a continuous proteoglycan- and type II collagen rich ECM for BM-MSCs but not ADPCs. Therefore, this study showed both protein and gene expression evidence of superior chondrogenesis of BM-MSCs relative to ADPCs.

Original languageEnglish (US)
Pages (from-to)322-331
Number of pages10
JournalJournal of Orthopaedic Research
Volume26
Issue number3
DOIs
StatePublished - Mar 2008
Externally publishedYes

Fingerprint

Chondrogenesis
Hydrogel
Horses
Hydrogels
Mesenchymal Stromal Cells
Stem Cells
Bone Marrow
Proteoglycans
Aggrecans
Sepharose
Extracellular Matrix
Peptides
Collagen Type II
Gene Expression
Cartilage
Cell Culture Techniques
Chondrocytes
Polymerase Chain Reaction

Keywords

  • Chondrogenesis
  • Progenitor cells
  • Tissue engineering

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine

Cite this

Evaluation of adult equine bone marrow- and adipose-derived progenitor cell chondrogenesis in hydrogel cultures. / Kisiday, John D.; Kopesky, Paul W.; Evans, Christopher H; Grodzinsky, Alan J.; Mcllwraith, C. Wayne; Frisbie, David D.

In: Journal of Orthopaedic Research, Vol. 26, No. 3, 03.2008, p. 322-331.

Research output: Contribution to journalArticle

Kisiday, John D. ; Kopesky, Paul W. ; Evans, Christopher H ; Grodzinsky, Alan J. ; Mcllwraith, C. Wayne ; Frisbie, David D. / Evaluation of adult equine bone marrow- and adipose-derived progenitor cell chondrogenesis in hydrogel cultures. In: Journal of Orthopaedic Research. 2008 ; Vol. 26, No. 3. pp. 322-331.
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