Evaluation of a xenogeneic acellular collagen matrix as a small-diameter vascular graft in dogs-preliminary observations

Simona Nemcova, Audra A. Noel, Corey J. Jost, Peter Gloviczki, Virginia M Miller, Kelvin G M Brockbank

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Autogenous veins are the materials of choice for arterial reconstruction. In the absence of autogenous material, prosthetic materials are used. However, vascular prostheses of less than 0.4 cm in diameter have low long-term patency. This study was designed to determine if cells would infiltrate an engineered xenogeneic biomaterial used as a small-diameter arterial graft in dogs and, if so, to determine the phenotype of the infiltrating cells. Nine acellular xenogeneic grafts (0.4 cm in diameter, 5 cm long), composed of porcine collagen derived from the submucosa of the small intestine and type I bovine collagen, were implanted as end-to-end interposition grafts in femoral arteries of five male mongrel dogs (total of nine grafts). All dogs received daily aspirin (325 mg). Patency of implanted grafts was monitored weekly by Duplex ultrasonography. After 9 weeks, or earlier in case of blood flow reduction by at least 75%, grafts were explanted and prepared for light or electron microscopy to evaluate cellularization. Eight of nine grafts remained patent up to 9 weeks. At explant, diameters were 0.31 ± 0.02 cm at the midgraft, and 0.14 ± 0.01 and 0.19 ± 0.01 cm at the proximal and distal anastomoses. At explant, cells of mesenchymal origin (endothelial cells, smooth muscle cells, myofibroblasts) were embedded in the extracellular matrix of the graft scaffold. Minimal evidence of cellular inflammatory reaction and no aneurysmal dilatation or thrombus formation was detected. Variable degrees of hyperplasia were present at proximal and distal anastomoses. This preliminary study demonstrates that a collagen-based xenogeneic biomaterial provides a scaffold for cellularization when used for arterial reconstruction in dogs.

Original languageEnglish (US)
Pages (from-to)321-330
Number of pages10
JournalJournal of Investigative Surgery
Volume14
Issue number6
DOIs
StatePublished - 2001

Fingerprint

Blood Vessels
Collagen
Dogs
Transplants
Biocompatible Materials
Blood Vessel Prosthesis
Myofibroblasts
Femoral Artery
Collagen Type I
Aspirin
Small Intestine
Smooth Muscle Myocytes
Hyperplasia
Extracellular Matrix
Dilatation
Veins
Ultrasonography
Electron Microscopy
Thrombosis
Swine

Keywords

  • Arterial reconstruction
  • Biomaterial
  • Blood flow
  • Graft patency

ASJC Scopus subject areas

  • Surgery

Cite this

Evaluation of a xenogeneic acellular collagen matrix as a small-diameter vascular graft in dogs-preliminary observations. / Nemcova, Simona; Noel, Audra A.; Jost, Corey J.; Gloviczki, Peter; Miller, Virginia M; Brockbank, Kelvin G M.

In: Journal of Investigative Surgery, Vol. 14, No. 6, 2001, p. 321-330.

Research output: Contribution to journalArticle

Nemcova, Simona ; Noel, Audra A. ; Jost, Corey J. ; Gloviczki, Peter ; Miller, Virginia M ; Brockbank, Kelvin G M. / Evaluation of a xenogeneic acellular collagen matrix as a small-diameter vascular graft in dogs-preliminary observations. In: Journal of Investigative Surgery. 2001 ; Vol. 14, No. 6. pp. 321-330.
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