Evaluation of a real-time PCR assay for the detection of the klebsiella pneumoniae carbapenemase genes in microbiological samples in comparison with the modified hodge test

Aditya Raghunathan, Linoj Samuel, Robert J. Tibbetts

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

Transfer of the blaKPC genes encoding the Klebsiella pneumoniae carbapenemase (KPC) are increasingly responsible for emerging carbapenem resistance. The modified Hodge test (MHT) is recommended for the detection of KPC. We compared MHT with a real-time polymerase chain reaction (PCR) assay targeting common subtypes of blaKPC, using previously described forward and reverse primer sequences. The PCR product was detected using SYBR Green (Applied Biosystems, Foster City, CA) and confirmed by melt curve analysis. PCR was positive in 96% (52/54) of isolates that were MHT+, 90% (28/31) of MHT- isolates were PCR-, and the results were strongly correlated (P = 0001; Fisher exact test). The PCR assay is a sensitive, specific, and rapid test for detecting blaKPC genes. It could help optimize patient care by reducing the time taken to institute appropriate antimicrobial therapy and so help improve patient outcomes.

Original languageEnglish (US)
Pages (from-to)566-571
Number of pages6
JournalAmerican journal of clinical pathology
Volume135
Issue number4
DOIs
StatePublished - Apr 1 2011

Keywords

  • BlaKPC genes
  • KP.C.
  • Klebsiella pneumoniae carbapenemase
  • Modified Hodge test

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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