Evaluation of a genus-and group-specific rapid PCR assay panel on synovial fluid for diagnosis of prosthetic knee infection

Dante P. Melendez, Kerryl E. Greenwood-Quaintance, Elie F. Berbari, Douglas R. Osmon, Jayawant N. Mandrekar, Arlen D. Hanssen, Robin Patel

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

We evaluated a genus-and group-specific PCR assay panel using 284 prosthetic knee synovial fluid samples collected from patients presenting to our institution with implant failure. Using the Musculoskeletal Infection Society diagnostic criteria, 88 and 196 samples were classified as showing prosthetic joint infection (PJI) and aseptic failure (AF), respectively. Sensitivities of the synovial fluid PCR panel and culture were 55.6% and 76.1% (P<0.001), respectively, and specificities were 91.8% and 97.4% (P≤0.016), respectively. Among the 70 subjects who had received antibiotics within the month preceding synovial fluid aspiration (48 of whom had PJI), PCR panel and synovial fluid culture sensitivities were 64.5% and 85.4%, respectively (P<0.0001). In this group, the PCR panel detected Staphylococcus aureus in two culture-negative PJI cases. Overall, the evaluated molecular diagnostic tool had low sensitivity when applied to synovial fluid.

Original languageEnglish (US)
Pages (from-to)120-126
Number of pages7
JournalJournal of clinical microbiology
Volume54
Issue number1
DOIs
StatePublished - Jan 2016

ASJC Scopus subject areas

  • Microbiology (medical)

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