Evaluation and clinical correlations of bone marrow angiogenesis in myelofibrosis with myeloid metaplasia

R. A. Mesa, C. A. Hanson, S Vincent Rajkumar, G. Schroeder, Ayalew Tefferi

Research output: Contribution to journalArticle

199 Citations (Scopus)

Abstract

Recent observations have underscored the biologic relevance of intratumoral angiogenesis and its potential impact on prognosis. Increased bone marrow angiogenesis has been demonstrated in a variety of hematologic disorders, including multiple myeloma. The extent and prognostic significance of bone marrow angiogenesis in 114 patients with myelofibrosis with myeloid metaplasia (MMM) was investigated. A control group of 44 patients without bone marrow disease, 15 patients with polycythemia vera, and 17 patients with essential thrombocythemia was also studied. Bone marrow microvessel density was assessed by a semiquantitative method, visual microvessel grading, and 2 separate quantitative methods, visual count and computerized image analysis. Angiogenesis estimation by all 3 methods was highly comparable. On visual microvessel grading, a grade 3 or 4 increase in bone marrow angiogenesis was demonstrated in 70% of patients with MMM, 33% of patients with polycythemia vera, 12% of patients with essential thrombocythemia, and 0% of normal controls. In a multivariate analysis, increased anglogenesis in MMM correlated significantly with increased spleen size and was found to be a significant and independent risk factor for overall survival. Increases in marrow angiogenesis correlated with hypercellularity and megakaryocyte clumping. In contrast, these 2 features were inversely proportional to reticulin fibrosis, whereas increases in marrow angiogenesis were independent of reticulin fibrosis. These preliminary findings suggest that neo-angiogenesis is an integral component of the bone marrow stromal reaction in MMM and may provide useful prognostic information and a rationale for the therapeutic investigation of anti-angiogenic agents. (C) 2000 by The American Society of Hematology.

Original languageEnglish (US)
Pages (from-to)3374-3380
Number of pages7
JournalBlood
Volume96
Issue number10
StatePublished - Nov 15 2000

Fingerprint

Primary Myelofibrosis
Bone
Bone Marrow
Reticulin
Microvessels
Essential Thrombocythemia
Polycythemia Vera
Fibrosis
Bone Marrow Diseases
Megakaryocytes
Image analysis
Multiple Myeloma
Spleen
Multivariate Analysis
Control Groups
Survival

ASJC Scopus subject areas

  • Hematology

Cite this

Evaluation and clinical correlations of bone marrow angiogenesis in myelofibrosis with myeloid metaplasia. / Mesa, R. A.; Hanson, C. A.; Rajkumar, S Vincent; Schroeder, G.; Tefferi, Ayalew.

In: Blood, Vol. 96, No. 10, 15.11.2000, p. 3374-3380.

Research output: Contribution to journalArticle

@article{01aca39f7a4244a48c15c470adaf3b79,
title = "Evaluation and clinical correlations of bone marrow angiogenesis in myelofibrosis with myeloid metaplasia",
abstract = "Recent observations have underscored the biologic relevance of intratumoral angiogenesis and its potential impact on prognosis. Increased bone marrow angiogenesis has been demonstrated in a variety of hematologic disorders, including multiple myeloma. The extent and prognostic significance of bone marrow angiogenesis in 114 patients with myelofibrosis with myeloid metaplasia (MMM) was investigated. A control group of 44 patients without bone marrow disease, 15 patients with polycythemia vera, and 17 patients with essential thrombocythemia was also studied. Bone marrow microvessel density was assessed by a semiquantitative method, visual microvessel grading, and 2 separate quantitative methods, visual count and computerized image analysis. Angiogenesis estimation by all 3 methods was highly comparable. On visual microvessel grading, a grade 3 or 4 increase in bone marrow angiogenesis was demonstrated in 70{\%} of patients with MMM, 33{\%} of patients with polycythemia vera, 12{\%} of patients with essential thrombocythemia, and 0{\%} of normal controls. In a multivariate analysis, increased anglogenesis in MMM correlated significantly with increased spleen size and was found to be a significant and independent risk factor for overall survival. Increases in marrow angiogenesis correlated with hypercellularity and megakaryocyte clumping. In contrast, these 2 features were inversely proportional to reticulin fibrosis, whereas increases in marrow angiogenesis were independent of reticulin fibrosis. These preliminary findings suggest that neo-angiogenesis is an integral component of the bone marrow stromal reaction in MMM and may provide useful prognostic information and a rationale for the therapeutic investigation of anti-angiogenic agents. (C) 2000 by The American Society of Hematology.",
author = "Mesa, {R. A.} and Hanson, {C. A.} and Rajkumar, {S Vincent} and G. Schroeder and Ayalew Tefferi",
year = "2000",
month = "11",
day = "15",
language = "English (US)",
volume = "96",
pages = "3374--3380",
journal = "Blood",
issn = "0006-4971",
publisher = "American Society of Hematology",
number = "10",

}

TY - JOUR

T1 - Evaluation and clinical correlations of bone marrow angiogenesis in myelofibrosis with myeloid metaplasia

AU - Mesa, R. A.

AU - Hanson, C. A.

AU - Rajkumar, S Vincent

AU - Schroeder, G.

AU - Tefferi, Ayalew

PY - 2000/11/15

Y1 - 2000/11/15

N2 - Recent observations have underscored the biologic relevance of intratumoral angiogenesis and its potential impact on prognosis. Increased bone marrow angiogenesis has been demonstrated in a variety of hematologic disorders, including multiple myeloma. The extent and prognostic significance of bone marrow angiogenesis in 114 patients with myelofibrosis with myeloid metaplasia (MMM) was investigated. A control group of 44 patients without bone marrow disease, 15 patients with polycythemia vera, and 17 patients with essential thrombocythemia was also studied. Bone marrow microvessel density was assessed by a semiquantitative method, visual microvessel grading, and 2 separate quantitative methods, visual count and computerized image analysis. Angiogenesis estimation by all 3 methods was highly comparable. On visual microvessel grading, a grade 3 or 4 increase in bone marrow angiogenesis was demonstrated in 70% of patients with MMM, 33% of patients with polycythemia vera, 12% of patients with essential thrombocythemia, and 0% of normal controls. In a multivariate analysis, increased anglogenesis in MMM correlated significantly with increased spleen size and was found to be a significant and independent risk factor for overall survival. Increases in marrow angiogenesis correlated with hypercellularity and megakaryocyte clumping. In contrast, these 2 features were inversely proportional to reticulin fibrosis, whereas increases in marrow angiogenesis were independent of reticulin fibrosis. These preliminary findings suggest that neo-angiogenesis is an integral component of the bone marrow stromal reaction in MMM and may provide useful prognostic information and a rationale for the therapeutic investigation of anti-angiogenic agents. (C) 2000 by The American Society of Hematology.

AB - Recent observations have underscored the biologic relevance of intratumoral angiogenesis and its potential impact on prognosis. Increased bone marrow angiogenesis has been demonstrated in a variety of hematologic disorders, including multiple myeloma. The extent and prognostic significance of bone marrow angiogenesis in 114 patients with myelofibrosis with myeloid metaplasia (MMM) was investigated. A control group of 44 patients without bone marrow disease, 15 patients with polycythemia vera, and 17 patients with essential thrombocythemia was also studied. Bone marrow microvessel density was assessed by a semiquantitative method, visual microvessel grading, and 2 separate quantitative methods, visual count and computerized image analysis. Angiogenesis estimation by all 3 methods was highly comparable. On visual microvessel grading, a grade 3 or 4 increase in bone marrow angiogenesis was demonstrated in 70% of patients with MMM, 33% of patients with polycythemia vera, 12% of patients with essential thrombocythemia, and 0% of normal controls. In a multivariate analysis, increased anglogenesis in MMM correlated significantly with increased spleen size and was found to be a significant and independent risk factor for overall survival. Increases in marrow angiogenesis correlated with hypercellularity and megakaryocyte clumping. In contrast, these 2 features were inversely proportional to reticulin fibrosis, whereas increases in marrow angiogenesis were independent of reticulin fibrosis. These preliminary findings suggest that neo-angiogenesis is an integral component of the bone marrow stromal reaction in MMM and may provide useful prognostic information and a rationale for the therapeutic investigation of anti-angiogenic agents. (C) 2000 by The American Society of Hematology.

UR - http://www.scopus.com/inward/record.url?scp=0034670052&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034670052&partnerID=8YFLogxK

M3 - Article

C2 - 11071630

AN - SCOPUS:0034670052

VL - 96

SP - 3374

EP - 3380

JO - Blood

JF - Blood

SN - 0006-4971

IS - 10

ER -