Evaluating yeast biosynthetic vacuolar transport

Brian A. Davies, Darren S. Carney, Bruce F. Horazdovsky

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Study of the lysosomal protein transport system has been facilitated through dissection of the analogous vacuolar protein sorting (VPS) pathway in Saccharomyces cerevisiae. Resident enzymes of the yeast vacuole are synthesized as inactive precursors and are cleaved to their mature forms upon delivery to this compartment. Quantitative assessment of this delivery can be achieved through the use of pulse-chase experiments monitoring the cleavage of zymogens to their mature forms. The experimental procedures for analysis of carboxypeptidase Y (CPY) and carboxypeptidase S (CPS) maturation are described.

Original languageEnglish (US)
Title of host publicationProtein Targeting Protocols, Second Edition
Pages329-337
Number of pages9
DOIs
StatePublished - May 21 2007

Publication series

NameMethods in Molecular Biology
Volume390
ISSN (Print)1064-3745

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Keywords

  • CPS
  • CPY
  • Carboxypeptidase S
  • Carboxypeptidase Y
  • ESCRT
  • Endosome
  • MVB
  • VPS
  • Vacuolar protein sorting
  • Yeast vacuole

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Davies, B. A., Carney, D. S., & Horazdovsky, B. F. (2007). Evaluating yeast biosynthetic vacuolar transport. In Protein Targeting Protocols, Second Edition (pp. 329-337). (Methods in Molecular Biology; Vol. 390). https://doi.org/10.1385/1-59745-466-4:329