Evaluating yeast biosynthetic vacuolar transport

Brian A. Davies, Darren S. Carney, Bruce F Horazdovsky

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Study of the lysosomal protein transport system has been facilitated through dissection of the analogous vacuolar protein sorting (VPS) pathway in Saccharomyces cerevisiae. Resident enzymes of the yeast vacuole are synthesized as inactive precursors and are cleaved to their mature forms upon delivery to this compartment. Quantitative assessment of this delivery can be achieved through the use of pulse-chase experiments monitoring the cleavage of zymogens to their mature forms. The experimental procedures for analysis of carboxypeptidase Y (CPY) and carboxypeptidase S (CPS) maturation are described.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
Pages329-337
Number of pages9
Volume390
DOIs
StatePublished - May 21 2007

Publication series

NameMethods in Molecular Biology
Volume390
ISSN (Print)10643745

Fingerprint

Protein Transport
Yeasts
Cathepsin A
Carboxypeptidases
Enzyme Precursors
Vacuoles
Saccharomyces cerevisiae
Dissection
Enzymes
lysosomal proteins

Keywords

  • Carboxypeptidase S
  • Carboxypeptidase Y
  • CPS
  • CPY
  • Endosome
  • ESCRT
  • MVB
  • Vacuolar protein sorting
  • VPS
  • Yeast vacuole

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology

Cite this

Davies, B. A., Carney, D. S., & Horazdovsky, B. F. (2007). Evaluating yeast biosynthetic vacuolar transport. In Methods in Molecular Biology (Vol. 390, pp. 329-337). (Methods in Molecular Biology; Vol. 390). https://doi.org/10.1385/1-59745-466-4:329

Evaluating yeast biosynthetic vacuolar transport. / Davies, Brian A.; Carney, Darren S.; Horazdovsky, Bruce F.

Methods in Molecular Biology. Vol. 390 2007. p. 329-337 (Methods in Molecular Biology; Vol. 390).

Research output: Chapter in Book/Report/Conference proceedingChapter

Davies, BA, Carney, DS & Horazdovsky, BF 2007, Evaluating yeast biosynthetic vacuolar transport. in Methods in Molecular Biology. vol. 390, Methods in Molecular Biology, vol. 390, pp. 329-337. https://doi.org/10.1385/1-59745-466-4:329
Davies BA, Carney DS, Horazdovsky BF. Evaluating yeast biosynthetic vacuolar transport. In Methods in Molecular Biology. Vol. 390. 2007. p. 329-337. (Methods in Molecular Biology). https://doi.org/10.1385/1-59745-466-4:329
Davies, Brian A. ; Carney, Darren S. ; Horazdovsky, Bruce F. / Evaluating yeast biosynthetic vacuolar transport. Methods in Molecular Biology. Vol. 390 2007. pp. 329-337 (Methods in Molecular Biology).
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