EUS-guided ethanol injection of normal porcine pancreas: A pilot study

Harry Aslanian, Ronald R. Salem, Celia Marginean, Marie Robert, Jeffrey H. Lee, Mark Topazian

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Background: Ethanol injection is a potential means of EUS-guided pancreatic tissue ablation. The effects of injected ethanol on normal pancreas are unknown. Methods: Transgastric ethanol injection of the body and the tail of normal porcine pancreas was performed under EUS guidance in 8 anesthetized Yorkshire pigs: 98% ethanol was injected in 4 animals, and 50% ethanol was injected in 4 animals. Serum amylase levels were monitored, and EUS was repeated on day 7. Gross and histologic examination of the pancreas was performed on day 7. Results: All 8 animals tolerated ethanol injection without signs of distress. An increase in amylase levels was seen in all animals. Focal areas, 2 to 6 mm, of inflammation, necrosis, and fibrosis were observed after 50% ethanol injection. Larger (8-30 mm) areas of inflammation, necrosis, and fibrosis were seen after 98% ethanol injection, as well as one fluid collection and one colonic stricture. Conclusions: Injection of 50% ethanol into normal porcine pancreas results in focal inflammation, necrosis, and fibrosis at the injection site. Ethanol injection (98%) produces larger areas of inflammation, fibrosis, and necrosis, with local complications of pancreatitis.

Original languageEnglish (US)
Pages (from-to)723-727
Number of pages5
JournalGastrointestinal Endoscopy
Volume62
Issue number5
DOIs
StatePublished - Nov 1 2005

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Pancreas
Ethanol
Swine
Injections
Fibrosis
Necrosis
Inflammation
Amylases
Pancreatitis
Tail
Pathologic Constriction
Serum

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Gastroenterology

Cite this

EUS-guided ethanol injection of normal porcine pancreas : A pilot study. / Aslanian, Harry; Salem, Ronald R.; Marginean, Celia; Robert, Marie; Lee, Jeffrey H.; Topazian, Mark.

In: Gastrointestinal Endoscopy, Vol. 62, No. 5, 01.11.2005, p. 723-727.

Research output: Contribution to journalArticle

Aslanian, Harry ; Salem, Ronald R. ; Marginean, Celia ; Robert, Marie ; Lee, Jeffrey H. ; Topazian, Mark. / EUS-guided ethanol injection of normal porcine pancreas : A pilot study. In: Gastrointestinal Endoscopy. 2005 ; Vol. 62, No. 5. pp. 723-727.
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abstract = "Background: Ethanol injection is a potential means of EUS-guided pancreatic tissue ablation. The effects of injected ethanol on normal pancreas are unknown. Methods: Transgastric ethanol injection of the body and the tail of normal porcine pancreas was performed under EUS guidance in 8 anesthetized Yorkshire pigs: 98{\%} ethanol was injected in 4 animals, and 50{\%} ethanol was injected in 4 animals. Serum amylase levels were monitored, and EUS was repeated on day 7. Gross and histologic examination of the pancreas was performed on day 7. Results: All 8 animals tolerated ethanol injection without signs of distress. An increase in amylase levels was seen in all animals. Focal areas, 2 to 6 mm, of inflammation, necrosis, and fibrosis were observed after 50{\%} ethanol injection. Larger (8-30 mm) areas of inflammation, necrosis, and fibrosis were seen after 98{\%} ethanol injection, as well as one fluid collection and one colonic stricture. Conclusions: Injection of 50{\%} ethanol into normal porcine pancreas results in focal inflammation, necrosis, and fibrosis at the injection site. Ethanol injection (98{\%}) produces larger areas of inflammation, fibrosis, and necrosis, with local complications of pancreatitis.",
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AU - Topazian, Mark

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N2 - Background: Ethanol injection is a potential means of EUS-guided pancreatic tissue ablation. The effects of injected ethanol on normal pancreas are unknown. Methods: Transgastric ethanol injection of the body and the tail of normal porcine pancreas was performed under EUS guidance in 8 anesthetized Yorkshire pigs: 98% ethanol was injected in 4 animals, and 50% ethanol was injected in 4 animals. Serum amylase levels were monitored, and EUS was repeated on day 7. Gross and histologic examination of the pancreas was performed on day 7. Results: All 8 animals tolerated ethanol injection without signs of distress. An increase in amylase levels was seen in all animals. Focal areas, 2 to 6 mm, of inflammation, necrosis, and fibrosis were observed after 50% ethanol injection. Larger (8-30 mm) areas of inflammation, necrosis, and fibrosis were seen after 98% ethanol injection, as well as one fluid collection and one colonic stricture. Conclusions: Injection of 50% ethanol into normal porcine pancreas results in focal inflammation, necrosis, and fibrosis at the injection site. Ethanol injection (98%) produces larger areas of inflammation, fibrosis, and necrosis, with local complications of pancreatitis.

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