Ethanol Extract of Cissus quadrangularis Enhances Osteoblast Differentiation and Mineralization of Murine Pre-Osteoblastic MC3T3-E1 Cells

Raazia Tasadduq, Jonathan Gordon, Khalid A. Al-Ghanim, Jane B. Lian, Andre J. Van Wijnen, Janet L. Stein, Gary S. Stein, Abdul Rauf Shakoori

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Traditional medicinal literature and previous studies have reported the possible role of Cissus quadrangularis (CQ) as an anti-osteoporotic agent. This study examines the effectiveness of CQ in promoting osteoblast differentiation of the murine pre-osteoblast cell line, MC3T3-E1. Ethanolic extract of CQ (CQ-E) was found to affect growth kinetics of MC3T3-E1 cells in a dosage-dependent manner. High concentrations of CQ-E (more than 10 μg/ml) have particularly adverse effects, while lower concentrations of 0.1 and 1 µg/ml were non-toxic and did not affect cell viability. Notably, cell proliferation was significantly increased at the lower concentrations of CQ-E. CQ-E treatment also augmented osteoblast differentiation, as reflected by a substantial increase in expression of the early osteoblast marker ALP activity, and at later stage, by mineralization of extracellular matrix compared to the control group. These findings suggest dose-dependent effect of CQ-E with lower concentrations exhibiting anabolic and osteogenic properties. J. Cell. Physiol. 232: 540–547, 2017.

Original languageEnglish (US)
Pages (from-to)540-547
Number of pages8
JournalJournal of Cellular Physiology
Volume232
Issue number3
DOIs
StatePublished - Mar 1 2017

ASJC Scopus subject areas

  • Physiology
  • Clinical Biochemistry
  • Cell Biology

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    Tasadduq, R., Gordon, J., Al-Ghanim, K. A., Lian, J. B., Van Wijnen, A. J., Stein, J. L., Stein, G. S., & Shakoori, A. R. (2017). Ethanol Extract of Cissus quadrangularis Enhances Osteoblast Differentiation and Mineralization of Murine Pre-Osteoblastic MC3T3-E1 Cells. Journal of Cellular Physiology, 232(3), 540-547. https://doi.org/10.1002/jcp.25449